| Prolactin storage in a clonal strain of rat pituitary tumor cells is cell-cycle dependent. | |
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MedLine Citation:
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PMID: 7047539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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GH4C1 cells (CH cells) are a clonal strain of rat pituitary tumor cells which secrete prolactin. We measured intracellular prolactin at different stages of the cell cycle using flow microfluorometry. Prolactin was stained by an indirect immunocytochemical technique using fluorescein isothiocyanate (FITC)-conjugated antiserum, and DNA was stained simultaneously with propidium iodide. We found that prolactin storage in GH cells was cell-cycle dependent; prolactin storage increased as cells passed from G1 to S to G2 + M. We have shown previously that insulin and 17 beta-estradiol act synergistically to increase intracellular prolactin three- to sevenfold and slow the rate of cell growth to approximately 70% of control cells. In this study we observed that insulin and estradiol increased prolactin storage at each stage of the cell cycle but did not affect the cell-cycle distribution of the population even though cell growth was slowed. We conclude that insulin and estradiol did not increase prolactin storage by affecting the cell-cycle distribution of the population. |
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Authors:
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D R Kiino; D E Burger; P S Dannies |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of cell biology Volume: 93 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1982 May |
Date Detail:
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Created Date: 1982-09-10 Completed Date: 1982-09-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 459-62 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects Cell Line Clone Cells Estradiol / pharmacology Insulin / pharmacology Interphase* / drug effects Mitosis* / drug effects Pituitary Neoplasms Prolactin / metabolism* Rats |
| Grant Support | |
ID/Acronym/Agency:
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CA-16359/CA/NCI NIH HHS; HD-00272/HD/NICHD NIH HHS; HD-11487/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin; 50-28-2/Estradiol; 9002-62-4/Prolactin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 5 Year: 1982 Volume: 93 Issue: 2 First Page: 459 Last Page: 462 ID: 2112861 Publisher Id: 82239551 PubMed Id: 7047539 |
| Prolactin storage in a clonal strain of rat pituitary tumor cells is cell-cycle dependent | |
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