Document Detail


Prolactin receptor gene expression and immunolocalization of the prolactin receptor in human luteinized granulosa cells.
MedLine Citation:
PMID:  11675469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prolactin is mainly known for its role in breast development and lactation, but has been also implicated in other physiological functions such as immunoregulation and ovarian steroid production. Although prolactin and prolactin receptor (PRL-R) transcripts have been previously identified in the human ovary, the spatial localization of the receptor is unknown. To investigate the presence of PRL-R within the follicular apparatus, human luteinized granulosa cells were obtained at the time of follicular aspiration from women undergoing ovarian stimulation for IVF. RNA extracted from these cells was subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using specific primers for the PRL-R gene. In addition, paraffin sections of isolated granulosa cells and sections of premenopausal human ovaries were immunostained with a mouse anti-human PRL-R monoclonal antibody. PRL-R were immunolocalized to the cell membrane of isolated luteinized granulosa cells and PRL-R transcripts were detected in the extracted RNA. No detectable staining was noted in secondary and early antral follicles in archived paraffin sections. These findings confirm the presence of PRL-R in human luteinized granulosa cells and suggest a localized role for PRL within the mature follicle. The absence of PRL-R in the early follicle suggests that the effects of prolactin are exerted around the time of ovulation.
Authors:
N P Vlahos; E M Bugg; M J Shamblott; J Y Phelps; J D Gearhart; H A Zacur
Related Documents :
6241359 - Significance of the delta 5 and delta 4 steroidogenic pathways in the hamster preovulat...
14711789 - The modulatory role of transforming growth factor beta1 and androstenedione on follicle...
20067799 - The relationship between the production and the anti-gonadotrophic action of prostaglan...
1097499 - Endotoxin-stimulated spleen cells: characterization of the responding cells.
566619 - Hormonal regulation of ovarian cellular proliferation.
18276949 - A putative mitotic checkpoint dependent on mtor function controls cell proliferation an...
1502489 - Antrectomy does not accelerate reversal of omeprazole-induced trophic effects in the ra...
19071699 - A surface plasmon resonance biosensor for detecting pseudomonas aeruginosa cells with s...
3985989 - Monoamine oxidase in single nerve cell bodies from locus coeruleus of the rat. a microg...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular human reproduction     Volume:  7     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-24     Completed Date:  2002-02-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  1033-8     Citation Subset:  IM    
Affiliation:
Division of Reproductive Endocrinology, Department of Gynecology and Obstetrics, The Johns Hopkins Medical Institutions, 600 N Wolfe Street/Houck 246, Baltimore, MD 21287, USA. nvlahos@jhmi.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, Differentiation, Myelomonocytic / metabolism
Female
Gene Expression Regulation
Granulosa Cells / physiology*
Humans
Luteal Phase
Ovary / physiology
Ovulation Induction
Receptors, Prolactin / genetics*,  immunology,  metabolism*
Chemical
Reg. No./Substance:
0/Antigens, Differentiation, Myelomonocytic; 0/Receptors, Prolactin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Expression of a Y-box protein, YB2/RYB-a, precedes protamine 2 expression during spermatogenesis in ...
Next Document:  Expression of a green fluorescent protein variant in mouse oocytes by injection of RNA with an added...