| Prolactin-producing cells differentiate from G0/G1-arrested somatotrophs in vitro: an analysis of cell cycle phases and mammotroph differentiation. | |
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MedLine Citation:
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PMID: 10395227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to analyze the relationship between cell proliferation and mammotroph differentiation, we studied a somatotrophic cell line, MtT/S. MtT/S cell is known to differentiate into PRL-producing cells in response to stimulation with insulin or insulin-like growth factor-1 (IGF-1). Double immunostaining for bromodeoxyuridine (BrdU), which labels proliferating cells, and for GH or PRL showed that most BrdU-labeled cells were GH-immunopositive, whereas considerably few PRL-positive cells were labeled with BrdU. This was confirmed by immunostaining of proliferating cells with antibody to proliferating cell nuclear antigen (PCNA). Furthermore, flow-cytometry analysis indicated that most of the PRL-producing cells were in the G0/G1 phase of the cell cycle. In order to determine whether cell cycle changes are required for transdifferentiation of PRL-producing cells, MtT/S cells were cultivated in serum restricted medium for 7 days to reduce their mitotic activity and then treated with insulin and epidermal growth factor (EGF). Under these conditions, the cell cycle of MtT/S cells was significantly delayed, but the percentage of PRL-producing cells induced was almost identical to that under control conditions, showing that mitosis is not required for PRL- producing cell differentiation. We also labeled MtT/S cells with BrdU for 24 h during PRL-producing cell induction by insulin and EGF, and as a result BrdU-labeled proliferative cells were specifically absent from PRL-producing cell populations. These data, taken as whole, suggest that PRL cells differentiated from G0/G1 arrested somatotrophs and the PRL cells which appeared had their cell proliferation activity significantly declined. In conclusion, this is the first report showing the relationship cell between proliferation and differentiation of PRL cells. |
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Authors:
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H Goda; T Sakai; M Kurosumi; K Inoue |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrine journal Volume: 45 ISSN: 0918-8959 ISO Abbreviation: Endocr. J. Publication Date: 1998 Dec |
Date Detail:
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Created Date: 1999-08-24 Completed Date: 1999-08-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9313485 Medline TA: Endocr J Country: JAPAN |
Other Details:
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Languages: eng Pagination: 725-35 Citation Subset: IM |
Affiliation:
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Department of Regulation Biology, Faculty of Science, Saitama University, Urawa, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bromodeoxyuridine Cell Cycle* Cell Differentiation Cell Division Flow Cytometry Immunohistochemistry Interphase Microscopy, Confocal Pituitary Gland / cytology*, metabolism Prolactin / metabolism* Rats Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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59-14-3/Bromodeoxyuridine; 9002-62-4/Prolactin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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