Document Detail

Prolactin induces regional vasoconstriction through the beta2-adrenergic and nitric oxide mechanisms.
MedLine Citation:
PMID:  17463060     Owner:  NLM     Status:  MEDLINE    
Prolactin has been associated with many effects and has been implicated in the pathogenesis of pregnancy-related hypertensive disorders, although little is known about its vascular effects. The present study was designed to determine the primary effect of prolactin on regional vascular beds and the mechanisms involved. In 37 anesthetized pigs, the infusion of 0.17 mug/kg min of prolactin at constant heart rate and arterial pressure decreased coronary, mesenteric, renal, and iliac blood flow. This response was graded in further five pigs by increasing the infused dose of the hormone between 0.017 and 1 mug/kg min. In 22 of the 37 pigs, blockade of cholinergic receptors (five pigs) and of alpha-adrenoceptors (five pigs) did not affect the prolactin-induced vascular response, which was abolished by blockade of beta(2)-adrenoceptors (five pigs) and by blockade of vascular nitric oxide (NO) synthase (seven pigs). In 15 of the 37 pigs the increases in measured blood flows caused by iv infusion of isoproterenol (five pigs) and by intraarterial administration of acetylcholine (five pigs) and of sodium nitroprusside (five pigs) were significantly reduced by infusion of prolactin. Moreover, the treatment of porcine aortic endothelial cells by prolactin caused a reduction of NO production and of the phosphorylation of ERK, Akt, and p38, which was prevented by the concomitant treatment by the beta(2)-adrenergic agonist albuterol. The present study showed that iv infusion of prolactin primarily caused coronary, mesenteric, renal, and iliac vasoconstriction. These effects were brought about by the inhibition of a vasodilatory beta(2)-adrenergic receptor-mediated effect related to the NO intracellular pathway.
Claudio Molinari; Elena Grossini; David A S G Mary; Francesca Uberti; Ezio Ghigo; Flavio Ribichini; Nicola Surico; Giovanni Vacca
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-26
Journal Detail:
Title:  Endocrinology     Volume:  148     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-19     Completed Date:  2007-09-11     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4080-90     Citation Subset:  AIM; IM    
Laboratorio di Fisiologia, Dipartimento di Medicina Clinica e Sperimentale, Facoltà di Medicina e Chirurgia, Università del Piemonte Orientale A. Avogadro, via Solaroli 17, I-28100 Novara, Italy.
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MeSH Terms
1-Phosphatidylinositol 3-Kinase / metabolism
Adrenergic beta-Agonists / pharmacology
Adrenergic beta-Antagonists / pharmacology
Aorta / cytology,  physiology
Butoxamine / pharmacology
Cells, Cultured
Coronary Circulation / drug effects,  physiology
Dose-Response Relationship, Drug
Endothelial Cells / cytology,  physiology*
Enzyme Inhibitors / pharmacology
Isoproterenol / pharmacology
Mitogen-Activated Protein Kinases / metabolism
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide / metabolism*
Nitric Oxide Synthase / antagonists & inhibitors,  metabolism
Prolactin / metabolism*,  pharmacology
Receptors, Adrenergic, beta-2 / antagonists & inhibitors,  metabolism*
Renal Circulation / drug effects,  physiology
Splanchnic Circulation / drug effects,  physiology
Vasoconstriction / drug effects,  physiology*
p38 Mitogen-Activated Protein Kinases / metabolism
Reg. No./Substance:
0/Adrenergic beta-Agonists; 0/Adrenergic beta-Antagonists; 0/Enzyme Inhibitors; 0/Receptors, Adrenergic, beta-2; 10102-43-9/Nitric Oxide; 2922-20-5/Butoxamine; 50903-99-6/NG-Nitroarginine Methyl Ester; 7683-59-2/Isoproterenol; 9002-62-4/Prolactin; EC Oxide Synthase; EC 3-Kinase; EC Protein Kinases; EC Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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