Document Detail


Prolactin and growth hormone induce differential cytokine and chemokine profile in murine peritoneal macrophages in vitro: involvement of p-38 MAP kinase, STAT3 and NF-kappaB.
MedLine Citation:
PMID:  18248819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of immune-neuroendocrine interactions in the autoimmune diseases is well recognized. Autoimmune rheumatoid diseases in their active phase have been characterized by high levels of prolactin (PRL) as well as proinflammatory cytokines which suggest a co-relationship between them. In the present study, we have investigated the profile of cytokines secreted by macrophages on treatment with PRL and growth hormone (GH) in vitro. Significantly enhanced production of cytokines IL-1beta, IL-12p40 and IFN-gamma was observed on treatment of macrophages with PRL or GH. However, higher doses of PRL (1000 ng/ml) induced the production of anti-inflammatory cytokine IL-10, with significant abrogation in production of proinflammatory cytokines. It is further observed that PRL and GH induced the production of chemokines MIP-1alpha and RANTES. PRL but not GH selectively induced significantly enhanced production of MCP-1 and IP-10. It is further shown that p38 MAP kinase, STAT3 and NF-kappaB could play a differential regulatory role in PRL or GH induced production of cytokines by macrophages.
Authors:
Ajit Sodhi; Anurag Tripathi
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Publication Detail:
Type:  Journal Article     Date:  2008-01-08
Journal Detail:
Title:  Cytokine     Volume:  41     ISSN:  1096-0023     ISO Abbreviation:  Cytokine     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-11     Completed Date:  2008-02-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  162-73     Citation Subset:  IM    
Affiliation:
School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, UP 221005, India. ajit.sodhi@lycos.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Chemokine CCL2 / biosynthesis
Chemokine CCL3 / biosynthesis
Chemokine CCL5 / biosynthesis
Chemokine CXCL10 / biosynthesis
Chemokines / biosynthesis*
Curcumin / pharmacology
Cytokines / biosynthesis*
Female
Growth Hormone / pharmacology*
I-kappa B Proteins / biosynthesis
Imidazoles / pharmacology
Interferon-gamma / biosynthesis
Interleukin-10 / biosynthesis
Interleukin-12 Subunit p40 / biosynthesis
Interleukin-1beta / biosynthesis
Kinetics
Macrophages, Peritoneal / drug effects*,  metabolism*
Male
Mice
Mice, Inbred BALB C
NF-kappa B / physiology*
Phosphorylation
Polymyxin B / pharmacology
Prolactin / pharmacology*
Pyridines / pharmacology
STAT3 Transcription Factor / physiology*
Tyrphostins / pharmacology
p38 Mitogen-Activated Protein Kinases / physiology*
Chemical
Reg. No./Substance:
0/4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole; 0/Ccl2 protein, mouse; 0/Chemokine CCL2; 0/Chemokine CCL3; 0/Chemokine CCL5; 0/Chemokine CXCL10; 0/Chemokines; 0/Cytokines; 0/I-kappa B Proteins; 0/Imidazoles; 0/Interleukin-12 Subunit p40; 0/Interleukin-1beta; 0/NF-kappa B; 0/Pyridines; 0/STAT3 Transcription Factor; 0/Stat3 protein, mouse; 0/Tyrphostins; 0/alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; 130068-27-8/Interleukin-10; 1404-26-8/Polymyxin B; 458-37-7/Curcumin; 82115-62-6/Interferon-gamma; 9002-62-4/Prolactin; 9002-72-6/Growth Hormone; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases

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