Document Detail

Prolactin (PRL) induction of cyclooxygenase 2 (COX2) expression and prostaglandin (PG) production in hamster Leydig cells.
MedLine Citation:
PMID:  21803118     Owner:  NLM     Status:  MEDLINE    
Serum prolactin (PRL) variations play a crucial role in the photoperiodic-induced testicular regression-recrudescence transition in hamsters. We have previously shown that cyclooxygenase 2 (COX2), a key enzyme in the biosynthesis of prostaglandins (PGs), is expressed mostly in Leydig cells of reproductively active hamsters with considerable circulating and pituitary levels of PRL. In this study, we describe a stimulatory effect of PRL on COX2/PGs in hamster Leydig cells, which is mediated by IL-1β and prevented by P38-MAPK and JAK2 inhibitors. Furthermore, by preparative isoelectric focusing (IEF), we isolated PRL charge analogues from pituitaries of active [isoelectric points (pI): 5.16, 4.61, and 4.34] and regressed (pI: 5.44) hamsters. More acidic PRL charge analogues strongly induced COX2 expression, while less acidic ones had no effect. Our studies suggest that PRL induces COX2/PGs in hamster Leydig cells through IL-1β and activation of P38-MAPK and JAK2. PRL microheterogeneity detected in active/inactive hamsters may be responsible for the photoperiodic variations of COX2 expression in Leydig cells.
María Eugenia Matzkin; Verónica Ambao; Mónica Herminia Carino; Soledad Paola Rossi; Lorena González; Daniel Turyn; Stella Campo; Ricardo Saúl Calandra; Mónica Beatriz Frungieri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-23
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  348     ISSN:  1872-8057     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-10-31     Completed Date:  2012-03-05     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  33-46     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Laboratorio de Esteroides, Instituto de Biología y Medicina Experimental, CONICET, Vuelta de Obligado 2490 (1428), Ciudad de Buenos Aires, Argentina.
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MeSH Terms
Cyclooxygenase 2 / genetics,  metabolism*
Gene Expression
Interleukin-1beta / metabolism
Janus Kinase 2 / antagonists & inhibitors,  metabolism
Leydig Cells / metabolism*
MAP Kinase Signaling System
Pituitary Gland / metabolism
Prolactin / pharmacology,  physiology*
Prostaglandins / biosynthesis*
Protein Isoforms / metabolism
Receptors, Interleukin / metabolism
Receptors, Prolactin / metabolism
Testis / cytology,  physiology
Testosterone / metabolism
p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors,  metabolism
Reg. No./Substance:
0/Interleukin-1beta; 0/Prostaglandins; 0/Protein Isoforms; 0/Receptors, Interleukin; 0/Receptors, Prolactin; 58-22-0/Testosterone; 9002-62-4/Prolactin; EC 2; EC Kinase 2; EC protein, mouse; EC Mitogen-Activated Protein Kinases

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