| Prokineticin-1: a novel mediator of the inflammatory response in third-trimester human placenta. | |
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MedLine Citation:
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PMID: 18372330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Prokineticin-1 (PK1) is a recently described protein with a wide range of functions, including tissue-specific angiogenesis, modulation of inflammatory responses, and regulation of hemopoiesis. The aim of this study was to investigate the localization and expression of PK1 and PK receptor-1 (PKR1), their signaling pathways, and the effect of PK1 on expression of the inflammatory mediators cyclooxygenase (COX)-2 and IL-8 in third-trimester placenta. PK1 and PKR1 were highly expressed in term placenta and immunolocalized to syncytiotrophoblasts, cytotrophoblasts, fetal endothelium, and macrophages. PK1 induced a time-dependent increase in expression of IL-8 and COX-2, which was significantly reduced by inhibitors of Gq, cSrc, epidermal growth factor receptor (EGFR), and MAPK kinase. Treatment of third-trimester placenta with 40 nm PK1 induced a rapid phosphorylation of cSrc, EGFR, and ERK1/2. Phosphorylation of ERK1/2 in response to PK1 was dependent on sequential phosphorylation of cSrc and EGFR. Using double-immunofluorescent immunohistochemistry, PKR1 colocalized with IL-8 and COX-2 in placenta. These data suggest that PK1 may have a novel role as a mediator of the inflammatory response in placenta. |
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Authors:
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Fiona C Denison; Sharon Battersby; Anne E King; Michael Szuber; Henry N Jabbour |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-27 |
Journal Detail:
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Title: Endocrinology Volume: 149 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-30 Completed Date: 2008-09-22 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 3470-7 Citation Subset: AIM; IM |
Affiliation:
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Department of Reproductive and Developmental Sciences, Centre for Reproductive Biology, University of Edinburgh, Edinburgh, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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metabolism Antigens, CD31 / metabolism Antigens, Differentiation, Myelomonocytic / metabolism Blotting, Western Cyclooxygenase 2 / metabolism Extracellular Signal-Regulated MAP Kinases / metabolism Female Gene Expression / drug effects Humans Immunohistochemistry Interleukin-8 / metabolism Keratins / metabolism Microscopy, Confocal Microscopy, Fluorescence Myometrium / metabolism Phosphorylation / drug effects Placenta / drug effects, metabolism* Pregnancy Pregnancy Trimester, Third* Receptor, Epidermal Growth Factor / metabolism Receptors, G-Protein-Coupled / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Vascular Endothelial Growth Factor, Endocrine-Gland-Derived / genetics, metabolism*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, CD31; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human; 0/Interleukin-8; 0/PROKR1 protein, human; 0/Receptors, G-Protein-Coupled; 0/Vascular Endothelial Growth Factor, Endocrine-Gland-Derived; 68238-35-7/Keratins; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 2.7.10.1/Receptor, Epidermal Growth Factor; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases |
| Comments/Corrections | |
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