Document Detail

Prokaryotic transport in electrohydrodynamic structures.
MedLine Citation:
PMID:  22476025     Owner:  NLM     Status:  Publisher    
When a high-voltage direct-current is applied to two beakers filled with water, a horizontal electrohydrodynamic (EHD) bridge forms between the two beakers. In this work we study the transport and behavior of bacterial cells added to an EHD bridge set-up. Organisms were added to one or to both beakers, and the transport of the cells through the bridge was monitored using optical and microbiological techniques. It is shown that Escherichia coli top10 (Invitrogen, Carlsbad, CA, USA) and bioluminescent E. coli YMC10 with a plasmid (pJE202) containing Vibrio fischeri genes can survive the exposure to an EHD liquid bridge set-up and the cells are drawn toward the anode due to their negative surface charge. Dielectrophoresis and hydrostatic forces are likely to be the cause for their transport in the opposite direction which was observed as well, but to a much lesser extent. Most E. coli YMC10 bacteria which passed the EHD bridge exhibited increased luminescent activity after 24 h. This can be explained by two likely mechanisms: nutrient limitation in the heavier inoculated vials and a 'survival of the strongest' mechanism.
A H Paulitsch-Fuchs; E C Fuchs; A D Wexler; F T Freund; L J Rothschild; A Cherukupally; G J W Euverink
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-04
Journal Detail:
Title:  Physical biology     Volume:  9     ISSN:  1478-3975     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197454     Medline TA:  Phys Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  026006     Citation Subset:  -    
Wetsus, Centre of Excellence for Sustainable Water Technology, Leeuwarden, The Netherlands.
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