| Proinflammatory reaction and cytoskeletal alterations in endothelial cells after shock wave exposure. | |
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MedLine Citation:
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PMID: 16984799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Although the effects on human organs by shock waves (SWs) induced by medical treatments or high-energy trauma are well recognized, little is known about the effects on the cellular level. Since blood vessel injury is a common finding after SW exposure, we assessed the in vitro effects of SWs on human umbilical vein endothelial cells (HUVECs). METHODS: An in vitro trauma model was used to expose HUVEC monolayers to focused SWs or to shock waves plus cavitation (SWC), a subsequent phenomenon that is often considered the main cause of SW vascular injury. RESULTS: SWs alone did not cause any changes in the studied variables. In contrast, HUVEC monolayers exposed to SWC exhibited discrete central lesions with extensive cell death. Cells peripheral to the main lesion area displayed disassembly of dense peripheral bands and formation of actin stress fibers, indicating increased intercellular gaps. Expression of P-selectin was enhanced 11-fold compared with controls, whereas expression of E-selectin and intercellular adhesion molecule 1 was enhanced 8-fold (p < .05) and 1.5-fold (p < .01), respectively. The latter responses were preceded by nuclear translocation of nuclear factor kappaB subunit p65 by 16% (p < .01). When compared with mechanically produced lesions used as controls, SWC lesions exhibited an impaired regeneration rate of the endothelial cell layer (p < .001). Redistribution of centrosomes toward the lesion borders was less effective in the SWC samples compared with the mechanically produced lesions (p < .01). CONCLUSIONS: SWC lesions were associated with a switch to an endothelial proinflammatory phenotype, with an impaired regeneration rate and changes in cytoskeletal functions. |
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Authors:
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Anders Sondén; Anne-Sofie M Johansson; Jan Palmblad; B Thomas Kjellström |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of investigative medicine : the official publication of the American Federation for Clinical Research Volume: 54 ISSN: 1081-5589 ISO Abbreviation: J. Investig. Med. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-09-20 Completed Date: 2007-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9501229 Medline TA: J Investig Med Country: Canada |
Other Details:
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Languages: eng Pagination: 262-71 Citation Subset: IM |
Affiliation:
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Department of Surgery, Söder Hospital, The Karolinska Institutet, Stockholm, Sweden. anders.sonden@sos.ki.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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analysis Cells, Cultured Cytoskeleton / chemistry, pathology, radiation effects* Endothelial Cells / pathology, radiation effects* High-Energy Shock Waves* Humans Inflammation / etiology* Intercellular Adhesion Molecule-1 / analysis NF-kappa B / physiology Tubulin / analysis |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/NF-kappa B; 0/Tubulin; 126547-89-5/Intercellular Adhesion Molecule-1 |
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