| Proinflammatory exosomes in bronchoalveolar lavage fluid of patients with sarcoidosis. | |
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MedLine Citation:
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PMID: 20880880 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Sarcoidosis is a systemic disease of unknown aetiology characterised by granuloma formation and the presence of interferon γ (IFNγ)-producing T cells that cause inflammation and tissue damage in multiple organs, especially the lung. Exosomes are nano-sized immunomodulatory vesicles of endosomal origin released from a diverse range of cells and are also found in physiological fluids including bronchoalveolar lavage fluid (BALF) from healthy individuals. OBJECTIVE: To investigate whether exosomes are enriched in the lungs of patients with sarcoidosis compared with healthy individuals and whether they could contribute to pathogenesis. DESIGN: BALF exosomes from patients with sarcoidosis (n=36) and healthy controls (n=14) were compared by electron microscopy, flow cytometry, western blot analysis and mass spectrometry. BALF exosomes were incubated with autologous peripheral blood mononuclear cells (PBMCs) or the human bronchial epithelial cell line 16HBE14o-. Cytokines were measured by ELISPOT and ELISA. RESULTS: BALF from patients with sarcoidosis showed increased levels of exosomes compared with healthy individuals. Exosomes from patients showed significantly higher expression of MHC class I and II, tetraspanins CD9, CD63 and CD81 as well as neuregulin-1, known to be associated with cancer progression. Furthermore, BALF exosomes from patients induced significantly higher IFNγ and interleukin (IL)-13 production in autologous PBMCs compared with healthy individuals and could also stimulate IL-8 production from epithelial cells. CONCLUSION: The results indicate for the first time a role for exosomes in human lung disease with possible contributions to the initiation and progression of inflammation in sarcoidosis. This suggests that exosomes may be a new potential target for the clinical treatment of lung diseases. |
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Authors:
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Khaleda R Qazi; Patricia Torregrosa Paredes; Benita Dahlberg; Johan Grunewald; Anders Eklund; Susanne Gabrielsson |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-29 |
Journal Detail:
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Title: Thorax Volume: 65 ISSN: 1468-3296 ISO Abbreviation: Thorax Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-22 Completed Date: 2010-11-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417353 Medline TA: Thorax Country: England |
Other Details:
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Languages: eng Pagination: 1016-24 Citation Subset: IM |
Affiliation:
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Department of Medicine, Clinical Allergy Research Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bronchoalveolar Lavage Fluid / cytology* Cell Line Cells, Cultured Cytokines / biosynthesis Epithelial Cells / metabolism Exosomes / chemistry, physiology*, ultrastructure Female Humans Inflammation Mediators / metabolism Interleukin-8 / biosynthesis Leukocytes, Mononuclear / metabolism Male Middle Aged Proteins / analysis Sarcoidosis, Pulmonary / metabolism, pathology* Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Inflammation Mediators; 0/Interleukin-8; 0/Proteins |
| Comments/Corrections | |
Comment In:
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Thorax. 2010 Nov;65(11):947-8
[PMID:
20937642
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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