| Proinflammatory effects of Staphylococcus aureus exotoxin B on nasal epithelial cells. | |
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MedLine Citation:
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PMID: 16455372 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The aim of this study was to evaluate the effects of Staphylococcus aureus exotoxin B (SE-B) on proinflammatory cytokine/chemokine releases in primary nasal epithelial cell cultures (NECC) of subjects with and without chronic rhinosinusitis (CRS). STUDY DESIGN AND SETTING: NECC (CRS: n = 14; Controls: n = 11) were stimulated with SE-B. Protein concentrations of interleukin-(IL)-1beta, IL-6, and IL-8 levels were measured in NECC supernatants by ELISA before (T0) and after 24 hr stimulation with SE-B (T1). RESULTS: T0: supernatants of the NECC of CRS patients contained significant lower levels of IL-8 (2.1 ng/ml) compared to Controls (IL-8: 6.2 ng/ml; P < 0.01). T1: SE-B induced a significant increase of IL-6 in NECC (P < 0.001). IL-1beta was not detectable. CONCLUSIONS: This is the first study evaluating the effects of exotoxins on NECC. SE-B showed proinflammatory effects on NECC. SIGNIFICANCE: Our data suggest that resident NECC are involved in immunological responses to Staphylococcus aureus toxins, supplementing the so-called "superantigen hypothesis" in CRS. |
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Authors:
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Michael Damm; Gero Quante; Jakob Rosenbohm; Roland Rieckmann |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery Volume: 134 ISSN: 0194-5998 ISO Abbreviation: Otolaryngol Head Neck Surg Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-02-03 Completed Date: 2006-03-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8508176 Medline TA: Otolaryngol Head Neck Surg Country: United States |
Other Details:
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Languages: eng Pagination: 245-9 Citation Subset: IM |
Affiliation:
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Department of Otorhinolaryngology, Head and Neck Surgery, University of Cologne (Köln), Germany. michael.damm@uni-koeln.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Chronic Disease Culture Media, Serum-Free Epithelial Cells / drug effects* Exotoxins / pharmacology* Humans Interleukin-1 / analysis Interleukin-6 / analysis Interleukin-8 / analysis Nose / cytology* Rhinitis / metabolism* Sinusitis / metabolism* Staphylococcus aureus / immunology* Superantigens / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Serum-Free; 0/Exotoxins; 0/Interleukin-1; 0/Interleukin-6; 0/Interleukin-8; 0/Superantigens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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