Document Detail


Proinflammatory effects of Staphylococcus aureus exotoxin B on nasal epithelial cells.
MedLine Citation:
PMID:  16455372     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The aim of this study was to evaluate the effects of Staphylococcus aureus exotoxin B (SE-B) on proinflammatory cytokine/chemokine releases in primary nasal epithelial cell cultures (NECC) of subjects with and without chronic rhinosinusitis (CRS). STUDY DESIGN AND SETTING: NECC (CRS: n = 14; Controls: n = 11) were stimulated with SE-B. Protein concentrations of interleukin-(IL)-1beta, IL-6, and IL-8 levels were measured in NECC supernatants by ELISA before (T0) and after 24 hr stimulation with SE-B (T1). RESULTS: T0: supernatants of the NECC of CRS patients contained significant lower levels of IL-8 (2.1 ng/ml) compared to Controls (IL-8: 6.2 ng/ml; P < 0.01). T1: SE-B induced a significant increase of IL-6 in NECC (P < 0.001). IL-1beta was not detectable. CONCLUSIONS: This is the first study evaluating the effects of exotoxins on NECC. SE-B showed proinflammatory effects on NECC. SIGNIFICANCE: Our data suggest that resident NECC are involved in immunological responses to Staphylococcus aureus toxins, supplementing the so-called "superantigen hypothesis" in CRS.
Authors:
Michael Damm; Gero Quante; Jakob Rosenbohm; Roland Rieckmann
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery     Volume:  134     ISSN:  0194-5998     ISO Abbreviation:  Otolaryngol Head Neck Surg     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-03     Completed Date:  2006-03-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8508176     Medline TA:  Otolaryngol Head Neck Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  245-9     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology, Head and Neck Surgery, University of Cologne (Köln), Germany. michael.damm@uni-koeln.de
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Chronic Disease
Culture Media, Serum-Free
Epithelial Cells / drug effects*
Exotoxins / pharmacology*
Humans
Interleukin-1 / analysis
Interleukin-6 / analysis
Interleukin-8 / analysis
Nose / cytology*
Rhinitis / metabolism*
Sinusitis / metabolism*
Staphylococcus aureus / immunology*
Superantigens / pharmacology*
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Exotoxins; 0/Interleukin-1; 0/Interleukin-6; 0/Interleukin-8; 0/Superantigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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