Document Detail

Proinflammatory cytokines interact synergistically with epidermal growth factor to stimulate PGE2 production in amnion-derived cells.
MedLine Citation:
PMID:  1410528     Owner:  NLM     Status:  MEDLINE    
Recent evidence has implicated cytokines and growth factors in the initiation of parturition in women. In the present study, the amnion-derived cell line WISH was used to determine whether proinflammatory cytokines (interleukins 1 beta, 6, and 8, tumor necrosis factor-alpha, and granulocyte/macrophage colony stimulating factor) could amplify epidermal growth factor-induced prostaglandin E2 production. WISH cells were preincubated with cytokines (0.0001-10 ng/ml) for 60 min and then challenged with EGF (10 ng/ml) for 4 hrs after which PGE2 production was measured by radioimmunoassay. EGF, IL-1 beta and TNF-alpha alone caused a dose-dependent increase in PGE2 production, while IL-6, IL-8 and GM-CSF were ineffective over the dose range tested. When cells were preincubated with IL-1 beta or TNF-alpha, there was a dose-dependent potentiation of EGF-induced PGE2 production that was greater than the sum of EGF alone and IL-1 beta or TNF-alpha alone. In each case, the minimum dose of IL-1 beta or TNF-alpha which amplified EGF-induced PGE2 production was 0.1 ng/ml (p less than 0.05, Student's t-test). These data show that low concentrations of IL-1 beta or TNF-alpha may serve to amplify EGF-mediated PGE2 biosynthesis in amnion-derived cells and suggest that cytokines may modulate EGF function in responsive cells.
D A Kniss; P D Zimmerman; R H Fertel; J D Iams
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prostaglandins     Volume:  44     ISSN:  0090-6980     ISO Abbreviation:  Prostaglandins     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-11-17     Completed Date:  1992-11-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0320271     Medline TA:  Prostaglandins     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  237-44     Citation Subset:  IM    
Department of Obstetrics and Gynecology (Division of Maternal-Fetal Medicine), Ohio State University College of Medicine, Columbus 43210.
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MeSH Terms
Amnion / cytology,  drug effects*,  metabolism
Cell Line
Cytokines / pharmacology*
Dinoprostone / analysis,  biosynthesis*
Drug Synergism
Epidermal Growth Factor / pharmacology*
Inflammation / chemically induced*
Reg. No./Substance:
0/Cytokines; 363-24-6/Dinoprostone; 62229-50-9/Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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