| Proinflammatory cytokines are involved in the initiation of the abnormal matrix process in pseudoexfoliation syndrome/glaucoma. | |
| | |
MedLine Citation:
|
PMID: 20395431 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Pseudoexfoliation (PEX) syndrome, which is an age-related, generalized elastotic matrix process, currently represents the most common identifiable risk factor for open-angle glaucoma. Dysregulated expression of proinflammatory cytokines has been implicated in the initiation of various fibrotic disorders and in the pathophysiology of glaucoma. Here we investigated the presence, expression, regulation, and functional significance of proinflammatory cytokines in eyes with early and late stages of PEX syndrome/glaucoma in comparison with normal and glaucomatous control eyes using multiplex bead analysis, immunoassays, real-time PCR, Western blotting, immunohistochemistry, and cell culture models. Early stages of PEX syndrome were characterized by approximately threefold (P < 0.005) elevated interleukin (IL)-6 and IL-8 levels in the aqueous humor and a concomitant approximately twofold (P < 0.001) increase in mRNA expression levels in anterior segment tissues as compared with controls. In contrast, late stages of PEX syndrome/glaucoma did not differ significantly from controls. IL-6, IL-6 receptor, and phospho-signal transducer and activator of transcription 3 could be mainly localized to walls of iris vessels and to the nonpigmented epithelium of ciliary processes. IL-6 and IL-8 were significantly up-regulated by ciliary epithelial cells in response to hypoxia or oxidative stress in vitro, whereas IL-6, but not IL-8, induced the expression of transforming growth factor-beta1 and elastic fiber proteins. These findings support a role for a stress-induced, spatially, and temporally restricted subclinical inflammation in the onset of the fibrotic matrix process characteristic of PEX syndrome/glaucoma. |
| | |
Authors:
|
Matthias Zenkel; Piotr Lewczuk; Anselm Jünemann; Friedrich E Kruse; Gottfried O H Naumann; Ursula Schlötzer-Schrehardt |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-15 |
Journal Detail:
|
Title: The American journal of pathology Volume: 176 ISSN: 1525-2191 ISO Abbreviation: Am. J. Pathol. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-06-03 Completed Date: 2010-10-04 Revised Date: 2011-07-28 |
Medline Journal Info:
|
Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: United States |
Other Details:
|
Languages: eng Pagination: 2868-79 Citation Subset: AIM; IM |
Affiliation:
|
Department of Ophthalmology, University of Erlangen-Nürnberg, Schwabachanlage 6, D-91054 Erlangen, Germany. matthias.zenkel@uk-erlangen.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Aged, 80 and over Animals Aqueous Humor / immunology Cell Line Cytokines / genetics, immunology* Exfoliation Syndrome* / immunology, pathology, physiopathology Extracellular Matrix / metabolism, pathology* Eye / anatomy & histology, immunology, pathology, physiopathology Female Glaucoma, Open-Angle* / immunology, pathology, physiopathology Humans Interleukin-6 / genetics, immunology Interleukin-8 / genetics, immunology Male Middle Aged Molecular Sequence Data |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 0/Interleukin-6; 0/Interleukin-8 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: This Month in AJP.
Next Document: Abundant expression of HIV target cells and C-type lectin receptors in the foreskin tissue of young ...