Document Detail


Prohormone convertases 1/3, 2, furin and protein 7B2 (Secretogranin V) in endocrine cells of the human pancreas.
MedLine Citation:
PMID:  17959263     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prohormone convertases (PCs) are proteinases that cleave inactive prohormones to biologically active peptides. Seven PCs have been identified; two of them, PC1/3 and PC2, have only been localized in neuroendocrine (NE) tissues; a third, furin, in both endocrine and exocrine tissues. We have studied the immunoreactivity of PC1/3, PC2 and furin in the four major NE cell types of the human pancreas by using double immunofluorescence techniques. The study also included the expression of NE secretory protein 7B2 (secretogranin V), a member of the granin family, which influences the function of PC2. The results showed that the three PCs and 7B2 were expressed only in endocrine pancreas, furin also in exocrine cells. Insulin (B) cells harboured PC1/3 and PC2, but not furin. Glucagon (A) cells were immunoreactive to all three PCs; all glucagon cells expressed PC2, but one subpopulation showed PC1/3 immunoreactivity and another furin. Only a few somatostatin (D) cells contained PC2, but no other proconvertase. Pancreatic polypeptide (PP) cells were non-reactive to all three PCs. 7B2 occurred only in insulin and glucagon cells. A varying co-localization pattern was observed between PCs and between PCs and 7B2, with the exception of PC1/3 and furin which were not co-localized. In conclusion, our study shows that PCs are localized in insulin and glucagon cells and do seem to be important in these cell types for processing of hormone and other protein precursors, especially chromogranins, but for the two other major cell types probably other enzymes are of importance.
Authors:
Guida Maria Portela-Gomes; Lars Grimelius; Mats Stridsberg
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Publication Detail:
Type:  Journal Article     Date:  2007-09-19
Journal Detail:
Title:  Regulatory peptides     Volume:  146     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-21     Completed Date:  2008-05-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  117-24     Citation Subset:  IM    
Affiliation:
Department of Gastroenterology, Lisbon University, Portugal. portela_gomes@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Furin / genetics,  metabolism*
Humans
Islets of Langerhans / physiology*
Neuroendocrine Secretory Protein 7B2 / genetics,  metabolism*
Pancreas / cytology,  enzymology,  metabolism*
Proprotein Convertase 1 / genetics,  metabolism*
Proprotein Convertase 2 / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Neuroendocrine Secretory Protein 7B2; EC 3.4.21.75/Furin; EC 3.4.21.93/Proprotein Convertase 1; EC 3.4.21.94/Proprotein Convertase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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