Document Detail

Prohibitins are required for cancer cell proliferation and adhesion.
MedLine Citation:
PMID:  20856874     Owner:  NLM     Status:  MEDLINE    
Prohibitin 1 (PHB1) is a highly conserved protein that together with its homologue prohibitin 2 (PHB2) mainly localizes to the inner mitochondrial membrane. Although it was originally identified by its ability to inhibit G1/S progression in human fibroblasts, its role as tumor suppressor is debated. To determine the function of prohibitins in maintaining cell homeostasis, we generated cancer cell lines expressing prohibitin-directed shRNAs. We show that prohibitin proteins are necessary for the proliferation of cancer cells. Down-regulation of prohibitin expression drastically reduced the rate of cell division. Furthermore, mitochondrial morphology was not affected, but loss of prohibitins did lead to the degradation of the fusion protein OPA1 and, in certain cancer cell lines, to a reduced capability to exhibit anchorage-independent growth. These cancer cells also exhibited reduced adhesion to the extracellular matrix. Taken together, these observations suggest prohibitins play a crucial role in adhesion processes in the cell and thereby sustaining cancer cell propagation and survival.
Claudia Sievers; Gwendolyn Billig; Kathleen Gottschalk; Thomas Rudel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-14
Journal Detail:
Title:  PloS one     Volume:  5     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2010  
Date Detail:
Created Date:  2010-09-21     Completed Date:  2011-02-18     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e12735     Citation Subset:  IM    
Research Group for Molecular Infection and Cancer Biology, Department of Molecular Biology, Max-Planck Institute for Infection Biology, Berlin, Germany.
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MeSH Terms
Cell Adhesion
Cell Division
Cell Line, Tumor
Cell Proliferation*
HEK293 Cells
HT29 Cells
HeLa Cells
Neoplasms / genetics,  metabolism,  physiopathology*
Repressor Proteins / genetics,  metabolism*
Reg. No./Substance:
0/Repressor Proteins; 0/prohibitin

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