Document Detail


Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells.
MedLine Citation:
PMID:  22573885     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The endoplasmic reticulum (ER) is both structurally and functionally complex, consisting of a dynamic network of interconnected sheets and tubules. To achieve a more comprehensive view of ER organization in interphase and mitotic cells and to address a discrepancy in the field (i.e., whether ER sheets persist, or are transformed to tubules, during mitosis), we analyzed the ER in four different mammalian cell lines using live-cell imaging, high-resolution electron microscopy, and three dimensional electron microscopy. In interphase cells, we found great variation in network organization and sheet structures among different cell lines. In mitotic cells, we show that the ER undergoes both spatial reorganization and structural transformation of sheets toward more fenestrated and tubular forms. However, the extent of spatial reorganization and sheet-to-tubule transformation varies among cell lines. Fenestration and tubulation of the ER correlates with a reduced number of membrane-bound ribosomes.
Authors:
Maija Puhka; Merja Joensuu; Helena Vihinen; Ilya Belevich; Eija Jokitalo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-09
Journal Detail:
Title:  Molecular biology of the cell     Volume:  23     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-29     Completed Date:  2012-11-05     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2424-32     Citation Subset:  IM    
Affiliation:
Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cell Line, Tumor
Cercopithecus aethiops
Cricetinae
Endoplasmic Reticulum / metabolism,  physiology*,  ultrastructure*
Humans
Mitosis*
Models, Biological
Ribosomes / metabolism
Time-Lapse Imaging
Vero Cells
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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