| Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells. | |
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MedLine Citation:
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PMID: 22573885 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The endoplasmic reticulum (ER) is both structurally and functionally complex, consisting of a dynamic network of interconnected sheets and tubules. To achieve a more comprehensive view of ER organization in interphase and mitotic cells and to address a discrepancy in the field (i.e., whether ER sheets persist, or are transformed to tubules, during mitosis), we have analyzed the ER in four different cell lines using live cell imaging, high-resolution electron microscopy, and 3D-electron microscopy. In interphase cells, we found great variation in network organization and sheet structures between different cell lines. In mitotic cells, we show that the ER undergoes both spatial reorganization and structural transformation of sheets toward more fenestrated and tubular forms. However, the extent of spatial reorganization and sheet-to-tubule transformation varies between cell lines. Fenestration and tubulation of the ER correlates with a reduced number of membrane-bound ribosomes. |
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Authors:
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Maija Puhka; Merja Joensuu; Helena Vihinen; Ilya Belevich; Eija Jokitalo |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-9 |
Journal Detail:
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Title: Molecular biology of the cell Volume: - ISSN: 1939-4586 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Institute of Biotechnology, Electron Microscopy Unit, University of Helsinki, 00014 Helsinki, Finland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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