Document Detail


Progress of O-acetylation and cross-linking of peptidoglycan in Neisseria gonorrhoeae grown in the presence of penicillin.
MedLine Citation:
PMID:  3117968     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The synthesis, cross-linking and O-acetylation of gonococcal peptidoglycan in growing cells were studied by following incorporation of radioactive glucosamine and separation of the SDS-insoluble peptidoglycan into uncross-linked (monomer) and cross-linked (dimer and oligomer) fragments. Cultures to which penicillin or piperacillin at concentrations near the minimum growth inhibitory concentration (MIC) had been added 20 min before the radioactive label were compared with controls. The beta-lactams affected the early stage of cross-linking (up to 3 min) but had no effect thereafter. The deficit of cross-linking, however, did not recover. The O-acetylation, particularly of the monomer fraction, was decreased by beta-lactams even at concentrations that had no effect on culture optical density, viable counts or overall peptidoglycan synthesis. These effects on O-acetylation occurred mainly after the first 3 min of incorporation, rather than before. O-Acetylation of the oligomer fraction was also followed. Here penicillin led to increased levels of O-acetylation during the early stages of incorporation but the final value was never exceeded; indeed at higher drug concentrations the later stages of O-acetylation of oligomers were inhibited (e.g. almost completely at 2.5 X MIC).
Authors:
A L Lear; H R Perkins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of general microbiology     Volume:  133     ISSN:  0022-1287     ISO Abbreviation:  J. Gen. Microbiol.     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-12-17     Completed Date:  1987-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375371     Medline TA:  J Gen Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1743-50     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Liverpool, UK.
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Glucosamine / metabolism
Neisseria gonorrhoeae / drug effects,  growth & development*
Penicillin G / pharmacology
Penicillins / pharmacology*
Peptidoglycan / metabolism*
Piperacillin / pharmacology
Chemical
Reg. No./Substance:
0/Penicillins; 0/Peptidoglycan; 3416-24-8/Glucosamine; 61-33-6/Penicillin G; 61477-96-1/Piperacillin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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