| Programmed reduction of ABC transporter activity in sea urchin germline progenitors. | |
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MedLine Citation:
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PMID: 22274698 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ATP-binding cassette (ABC) transporters protect embryos and stem cells from mutagens and pump morphogens that control cell fate and migration. In this study, we measured dynamics of ABC transporter activity during formation of sea urchin embryonic cells necessary for the production of gametes, termed the small micromeres. Unexpectedly, we found small micromeres accumulate 2.32 times more of the ABC transporter substrates calcein-AM, CellTrace RedOrange, BoDipy-verapamil and BoDipy-vinblastine, than any other cell in the embryo, indicating a reduction in multidrug efflux activity. The reduction in small micromere ABC transporter activity is mediated by a pulse of endocytosis occurring 20-60 minutes after the appearance of the micromeres--the precursors of the small micromeres. Treating embryos with phenylarsine oxide, an inhibitor of endocytosis, prevents the reduction of transporter activity. Tetramethylrhodamine dextran and cholera toxin B uptake experiments indicate that micromeres have higher rates of bulk and raft-associated membrane endocytosis during the window of transporter downregulation. We hypothesized that this loss of efflux transport could be required for the detection of developmental signaling molecules such as germ cell chemoattractants. Consistent with this hypothesis, we found that the inhibition of ABCB and ABCC-types of efflux transporters disrupts the ordered distribution of small micromeres to the left and right coelomic pouches. These results point to tradeoffs between signaling and the protective functions of the transporters. |
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Authors:
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Joseph P Campanale; Amro Hamdoun |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 139 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-25 Completed Date: 2012-03-16 Revised Date: 2013-04-12 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 783-92 Citation Subset: IM |
Affiliation:
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Marine Biology Research Division, Scripps Institution of Oceanography, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0202, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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ATP-Binding Cassette Transporters
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metabolism* Animals Biological Transport Endocytosis / physiology Fluorescent Dyes / metabolism Germ Cells / cytology, metabolism* Sea Urchins / cytology*, embryology*, metabolism* Signal Transduction / physiology Stem Cells / cytology, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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HD058070/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes |
| Comments/Corrections | |
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