Document Detail

Programmed phosphorylation of histone H2AX precedes a phase of DNA double strand break (DSB)-independent synapsis in mouse meiosis.
MedLine Citation:
PMID:  23035256     Owner:  NLM     Status:  Publisher    
Accurate homolog synapsis during meiosis is essential for faithful chromosome segregation and formation of viable gametes. The finding of Spo11-dependent γH2AX formation during leptotene and data on mutant mice have led to the notion that synapsis in mammals depends on meiotic DNA double-stranded break (DSB) repair. A second wave of ATM and Rad3 related (ATR)-dependent γH2AX formation has been observed in Atm-null mice during zygotene, suggesting that this wave of phosphorylation also occurs in normal mice. Here I aimed to confirm and to analyse in deep this wave of phosphorylation. Immunostaining of spread spermatocytes shows that γH2AX appears on the short last axis stretches to pair within all the nuclei undergoing a specific substage of late zygotene and disappears in every spermatocyte immediately after pairing completion. This γH2AX signal colocalizes with ATR, is Spo11-independent and does not colocalizes with free DNA 3'-end labelling. I conclude that ATR/γH2AX asynapsis signalling at the end of zygotene belongs to a physiologically programmed pathway operating at a specific meiotic step and I propose that this pathway is involved in the triggering of a phase of DSB-independent chromosome pairing that leads to synapsis completion in normal mouse meiosis.
Josefa Blanco Rodriguez
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-3
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  -     ISSN:  1741-7899     ISO Abbreviation:  Reproduction     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
J Blanco Rodriguez, Cell Biology, The University of Valladolid, Valladolid, 47005, Spain.
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