Document Detail

Programmed changes of cell cycle regulators by serum deprivation regardless of skeletal myocyte differentiation.
MedLine Citation:
PMID:  9856354     Owner:  NLM     Status:  MEDLINE    
Permanent withdrawal of skeletal myoblasts from the cell cycle precedes differentiation. We examined the changes of protein levels of cell cycle regulators and the activities of CDKs in differentiating (by serum deprivation) or in differentiation-inhibited (by serum deprivation + TGF-beta1 treated) C2C12 skeletal myocytes. Regardless of differentiation, protein levels of most cyclins declined over time while this effect was delayed slightly by TGF-beta1 for cyclins D1 and F. Although the protein levels of CDKs gradually decreased over time, the activities of CDK2 and cdc2 decreased dramatically between 0 and 12 h after serum deprivation in both groups. Decreased CDK2 and cdc2 activities were temporally related to decreased DNA synthesis. While the p27KIP1 protein increased in both groups, the p21CIP1 protein increased only in differentiating cells. In vivo, protein expressions of cyclins, CDKs, and p21CIP1 were high in fetal, but almost undetectable in adult skeletal muscle. In contrast, the levels of p27KIP1 protein in skeletal muscle were high throughout development. Thus, changes of cell cycle regulators in differentiating C2C12 myocytes paralleled those during skeletal muscle development of mice. These results suggest that the rapid reduction of DNA synthesis and activities of CDKs slow reduction of protein levels of cyclins and CDKs, and that the increase of p27KIP1 are programmed mechanisms upon mitogen deprivation regardless of differentiation in skeletal myocytes.
A Frith-Terhune; K N Koh; W J Jin; K B Chung; S K Park; G Y Koh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecules and cells     Volume:  8     ISSN:  1016-8478     ISO Abbreviation:  Mol. Cells     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-03-04     Completed Date:  1999-03-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  KOREA    
Other Details:
Languages:  eng     Pagination:  637-46     Citation Subset:  IM    
Cardiac Regeneration Group, CRIP and Institute of Cardiovascular Research, Chonbuk National University Medical School, Chonju, Korea.
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MeSH Terms
Cell Cycle Proteins / drug effects*,  genetics,  metabolism
Cell Differentiation / drug effects,  genetics
Cell Line
Culture Media / pharmacology*
Cyclin-Dependent Kinase Inhibitor p21
Cyclin-Dependent Kinase Inhibitor p27
Cyclin-Dependent Kinases / drug effects,  metabolism
Cyclins / drug effects,  metabolism
DNA / biosynthesis,  drug effects
Fetus / metabolism
Microtubule-Associated Proteins / drug effects,  metabolism
Muscle, Skeletal / cytology,  drug effects*,  metabolism
Thymidine / metabolism
Transforming Growth Factor beta / pharmacology
Tritium / diagnostic use
Tumor Suppressor Proteins*
Reg. No./Substance:
0/Cdkn1a protein, mouse; 0/Cdkn1b protein, mouse; 0/Cell Cycle Proteins; 0/Culture Media; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Microtubule-Associated Proteins; 0/Transforming Growth Factor beta; 0/Tumor Suppressor Proteins; 10028-17-8/Tritium; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 50-89-5/Thymidine; 9007-49-2/DNA; EC Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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