Document Detail


Programmed cell death of the megagametophyte during post-germinative growth of white spruce (Picea glauca) seeds is regulated by reactive oxygen species and the ubiquitin-mediated proteolytic system.
MedLine Citation:
PMID:  20833629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The megagametophyte of white spruce (Picea glauca) seeds undergoes programmed cell death following seed germination. This process is characterized by distinct morphological and biochemical features, such as DNA fragmentation and the induction of proteases. Biphasic production of hydrogen peroxide was detected in the megagametophyte following seed germination. ROS scavengers or inhibitors of ROS production decreased caspase-like protease activity and slowed the progression of cell death. One catalase (CAT) of white spruce reacted with antibodies directed against cotton-seed CAT. The corresponding CAT gene was cloned and compared with the catalase genes of other plant species. The activity of the white spruce CAT enzyme was stimulated by tyrosine phosphorylation. The phosphorylated CAT was subjected to ubiquitination and degraded by the proteasome. Furthermore, the proteasome inhibitor MG132 inhibited the degradation of CAT and delayed cell death. These results suggest that the interplay of CAT and the ubiquitin-mediated proteolytic system is critical in the control of ROS production and subsequent cell death.
Authors:
Xu He; Allison R Kermode
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-09
Journal Detail:
Title:  Plant & cell physiology     Volume:  51     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-11     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1707-20     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, Canada V5A 1S6.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Apoptosis*
Catalase / genetics,  metabolism
Cloning, Molecular
Cysteine Proteinase Inhibitors / pharmacology
DNA, Plant / genetics
Germination
Hydrogen Peroxide / analysis
Leupeptins / pharmacology
Lipid Peroxidation
Molecular Sequence Data
Ovule / cytology*,  enzymology,  growth & development
Phosphorylation
Picea / enzymology,  genetics,  growth & development*
Proteasome Endopeptidase Complex / metabolism
Reactive Oxygen Species / metabolism
Seeds / cytology,  enzymology,  growth & development*
Sequence Alignment
Tyrosine / metabolism
Ubiquitin / metabolism*
Ubiquitination
Chemical
Reg. No./Substance:
0/Cysteine Proteinase Inhibitors; 0/DNA, Plant; 0/Leupeptins; 0/Reactive Oxygen Species; 0/Ubiquitin; 133407-82-6/benzyloxycarbonylleucyl-leucyl-leucine aldehyde; 55520-40-6/Tyrosine; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.6/Catalase; EC 3.4.25.1/Proteasome Endopeptidase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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