Document Detail

Programmed cell death in the developing somites is promoted by nerve growth factor via its p75(NTR) receptor.
MedLine Citation:
PMID:  11112333     Owner:  NLM     Status:  MEDLINE    
Neurotrophins control neuron number during development by promoting the generation and survival of neurons and by regulating programmed neuronal death. In the latter case, the cell death induced by nerve growth factor (NGF) in the developing chick retina is mediated by p75(NTR), the common neurotrophin receptor (J. M. Frade, A. Rodriguez-Tebar, and Y.-A. Barde, 1996, Nature 383, 166-168). Here we show that NGF also induces the programmed death of paraxial mesoderm cells in the developing somites. Both NGF and p75(NTR) are expressed in the somites of chick embryos at the time and the place of programmed cell death. Moreover, neutralizing the activity of endogenous NGF with a specific blocking antibody, or antagonizing NGF binding to p75(NTR) by the application of human NT-4/5, reduces the levels of apoptotic cell death in both the sclerotome and the dermamyotome by about 50 and 70%, respectively. Previous data have shown that Sonic hedgehog is necessary for the survival of differentiated somite cells. Consistent with this, Sonic hedgehog induces a decrease of NGF mRNA in somite explant cultures, thus showing the antagonistic effect of NGF and Sonic hedgehog with respect to somite cell survival. The regulation of programmed cell death by NGF/p75(NTR) in a mesoderm-derived tissue demonstrates the capacity of neurotrophins and their receptors to influence critical developmental processes both within and outside of the nervous system.
M L Cotrina; M González-Hoyuela; J A Barbas; A Rodríguez-Tébar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental biology     Volume:  228     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-02-02     Completed Date:  2001-02-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  326-36     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Instituto Cajal de Neurobiología, CSIC, Avenida Doctor Arce, 37, E-28002 Madrid, Spain.
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MeSH Terms
Apoptosis / drug effects,  physiology*
Chick Embryo
Ectoderm / cytology,  drug effects,  physiology
Embryonic and Fetal Development
Mesoderm / cytology,  physiology
Nerve Growth Factor / physiology*
Nerve Growth Factors / pharmacology
Nervous System / cytology,  drug effects,  embryology
Neuroprotective Agents / pharmacology
Organ Specificity
Receptor, Nerve Growth Factor
Receptor, trkA / physiology
Receptors, Nerve Growth Factor / physiology*
Reg. No./Substance:
0/Nerve Growth Factors; 0/Neuroprotective Agents; 0/Receptor, Nerve Growth Factor; 0/Receptors, Nerve Growth Factor; 143551-63-7/neurotrophin 4; 145172-44-7/neurotrophin 5; 9061-61-4/Nerve Growth Factor; EC, trkA

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