Document Detail


Programmed cell death (apoptosis) in cord blood lymphocytes.
MedLine Citation:
PMID:  9049787     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cord blood lymphocytes are functionally immature and have deficient immune responses. In order to determine whether the process of programmed cell death is distinct between cord blood and peripheral blood lymphocytes, we analyzed the expression of fas and bax (apoptosis promoting genes) and bcl-2 and bcl-xL (apoptosis inhibiting genes) at protein or mRNA levels using flow cytometry and quantitative PCR methods, respectively. The susceptibility of T cell subsets from cord blood and adult peripheral blood to undergo apoptosis following restimulation with anti-CD3 or anti-Fas monoclonal antibodies was also studied. We observed that cord blood T cell subsets expressed lower levels of Fas and Bcl-2, a low bcl-2/bax ratio, and higher bcl-xL compared to peripheral blood. Additionally, upon primary stimulation with anti-CD3, cord blood T cell subsets were more resistant to apoptosis compared to peripheral blood. In contrast, rechallenge of previously stimulated lymphocytes with anti-CD3 monoclonal antibody triggered apoptosis in a larger proportion of T cells from cord blood as compared to peripheral blood, whereas the number of T cells undergoing anti-Fas-induced programmed cell death were lower in cord blood compared to peripheral blood.
Authors:
S Aggarwal; A Gupta; S Nagata; S Gupta
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical immunology     Volume:  17     ISSN:  0271-9142     ISO Abbreviation:  J. Clin. Immunol.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-05-13     Completed Date:  1997-05-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102137     Medline TA:  J Clin Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  63-73     Citation Subset:  IM    
Affiliation:
Basic and Clinical Immunology, University of California, Irvine 92697, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD95 / biosynthesis
Apoptosis / immunology*
Fas Ligand Protein
Fetal Blood / cytology,  immunology*
Humans
Ligands
Membrane Glycoproteins / biosynthesis
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins c-bcl-2 / biosynthesis,  genetics
RNA, Messenger / biosynthesis
T-Lymphocyte Subsets / immunology*,  metabolism
bcl-2-Associated X Protein
Chemical
Reg. No./Substance:
0/Antigens, CD95; 0/BAX protein, human; 0/FASLG protein, human; 0/Fas Ligand Protein; 0/Ligands; 0/Membrane Glycoproteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/bcl-2-Associated X Protein

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