Document Detail


Prognostic value of cytosolic thymidine kinase activity as a marker of proliferation in breast cancer.
MedLine Citation:
PMID:  7705935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thymidine kinase (TK) is involved in DNA synthesis by the salvage pathway. In this study, thymidine kinase (TK) was determined in routinely prepared cytosols of primary tumors from 290 breast-cancer patients. Enzyme activity was measured using a radioenzymatic method optimized for detection of the fetal isoenzyme. High levels of TK (> or = 126 mU/mg protein) were positively associated with histological grade in both pre/peri-and post-menopausal patients. In pre/peri-menopausal patients, high concentrations of TK were also found more frequently in progesterone receptor (PgR)-negative tumors than in PgR-positive samples. In post-menopausal patients, high levels of TK were associated with large tumor size, estrogen receptor (ER) negativity and PgR negativity. In univariate analysis, high levels of TK were strongly associated with shorter overall survival in both pre/peri- (p = 0.001) and post-menopausal patients (p = 0.02). Pre/peri-menopausal patients whose tumors had high levels of TK also had an increased risk of relapse (p = 0.001). In multivariate analysis (including treatment protocol, patient age, lymph-node involvement, tumor size, histological grade, ER and PgR status), TK status was found to be an independent prognostic factor for recurrence-free survival in pre/peri-menopausal patients with a weight similar to that of PgR status. In post-menopausal patients, TK was the only factor selected for overall survival.
Authors:
S Romain; I J Christensen; O Chinot; I Balslev; C Rose; P M Martin; S M Thorpe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  61     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-05-09     Completed Date:  1995-05-09     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7-12     Citation Subset:  IM    
Affiliation:
Laboratoire d'Oncologie Biologique-Biopathologie Tissulaire, Faculté de Médecine Nord, Marseille, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Breast Neoplasms / enzymology*,  metabolism,  pathology
Cell Transformation, Neoplastic
Cytosol / enzymology
DNA, Neoplasm / biosynthesis
Female
Flow Cytometry
Humans
Isoenzymes / metabolism*
Middle Aged
Multivariate Analysis
Neoplasm Recurrence, Local / enzymology
Predictive Value of Tests
Prognosis
Thymidine Kinase / metabolism*
Tumor Markers, Biological / metabolism*
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Isoenzymes; 0/Tumor Markers, Biological; EC 2.7.1.21/Thymidine Kinase

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