Document Detail


Prognostic significance of expression of nm23-H1 and focal adhesion kinase in non-small cell lung cancer.
MedLine Citation:
PMID:  17549350     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
nm23-H1, a nucleoside diphosphate kinase (NDPK), enhances drug sensitivity and has antimetastatic activity, whereas focal adhesion kinase (FAK) is closely associated with cell migration and tumour spreading. The relationship between these two proteins, however, is not well elucidated. In this study, we investigate their correlation in patients with non-small cell lung cancer (NSCLC). Expressions of nm23-H1 and FAK were examined by reverse transcription-polymerase chain reaction and immunoblotting in surgical resections. The relationship between these two genes was assessed statistically. Patients were classified into four groups according to the expression of nm23-H1 and FAK by immunohistochemistry: FAK-negative/nm23-H1-positive, FAK-negative/nm23-H1-negative, FAK-positive/nm23-H1-positive and FAK-positive/nm23-H1-negative. Although the causal correlation is still uncertain, our results showed that protein expression of nm23-H1 was inversely correlated with that of FAK. The combined analysis of nm23-H1 and FAK protein expression in the same tumour specimens revealed that patients with FAK-negative/nm23-H1-positive tumours survived the longest, 56 months, among those with nm23-H1 and FAK features (P<0.001). Our data indicate that expressions of nm23-H1 and FAK are inversely correlated. These results suggest that the status of nm23-H1 and FAK protein expression may help in predicting the aggressive behavior of NSCLC. However, further studies are warranted to clarify the impact of FAK on the function of nm23-H1 as an anti-metastatic gene.
Authors:
Nan-Yung Hsu; Chih-Yi Chen; Chung-Ping Hsu; Tze-Yi Lin; Ming-Chih Chou; Shiow-Her Chiou; Kuan-Chih Chow
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  18     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-05     Completed Date:  2007-08-16     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  81-5     Citation Subset:  IM    
Affiliation:
Division of Chest Surgery, China Medical University Hospital, Taichung 40227, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Carcinoma, Non-Small-Cell Lung / enzymology*,  pathology,  therapy
Female
Focal Adhesion Kinase 1 / genetics,  metabolism*
Humans
Immunoenzyme Techniques
Lung Neoplasms / enzymology*,  pathology,  therapy
Lymphatic Metastasis / pathology
Male
Middle Aged
NM23 Nucleoside Diphosphate Kinases
Neoplasm Staging
Nucleoside-Diphosphate Kinase / genetics,  metabolism*
Prognosis
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Survival Rate
Tumor Markers, Biological / analysis*
Chemical
Reg. No./Substance:
0/NM23 Nucleoside Diphosphate Kinases; 0/RNA, Messenger; 0/Tumor Markers, Biological; EC 2.7.10.1/Focal Adhesion Kinase 1; EC 2.7.10.2/PTK2 protein, human; EC 2.7.4.6/NME1 protein, human; EC 2.7.4.6/Nucleoside-Diphosphate Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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