| Progestogens reduce thromboxane production by cultured human endothelial cells. | |
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MedLine Citation:
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PMID: 20443717 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objectives Progestogens have been poorly studied concerning their roles in endothelial physiology. Prostanoids are vasoactive compounds, such as thromboxane A2, a potent vasoconstrictor, and prostacyclin, a vasodilator. We examined the effects of two progestogens used clinically, progesterone and medroxyprogesterone acetate, on thromboxane A2 production by cultured human umbilical vein endothelial cells (HUVEC) and investigated the role of progesterone receptors and the enzymes involved in production of thromboxane A2 and prostacyclin. Methods Cells were exposed to 1-100 nmol/l of either progesterone or medroxyprogesterone acetate, and thromboxane A2 production was measured in culture medium by enzyme immunoassay. Gene expression of prostacyclin synthase and thromboxane synthase was analyzed by quantitative real-time polymerase chain reaction. Expression of prostacyclin synthase protein was analyzed by Western blot. Results Both progestogens decreased thromboxane A2 release after 24 h. Protein and gene expression of prostacyclin synthase were increased after exposure to both progestogens, without changes in thromboxane synthase expression. These effects induced by progestogens were mediated through progesterone receptors, since they were decreased in the presence of the progesterone receptor antagonist RU486. The cyclo-oxygenase-1 selective inhibitor reduced thromboxane release. Conclusion Progesterone and medroxyprogesterone acetate decreased HUVEC thromboxane release in a progesterone receptor-dependent manner, without changes in thromboxane synthase expression and enhanced prostacyclin synthase gene and protein expression. |
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Authors:
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P J Oviedo; A Sobrino; S Novella; C Rius; A Laguna-Fernandez; M A García-Pérez; J J Tarín; A Cano; C Hermenegildo |
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Publication Detail:
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Type: Journal Article Date: 2010-05-05 |
Journal Detail:
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Title: Climacteric : the journal of the International Menopause Society Volume: 13 ISSN: 1473-0804 ISO Abbreviation: Climacteric Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9810959 Medline TA: Climacteric Country: England |
Other Details:
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Languages: eng Pagination: 41-8 Citation Subset: IM |
Affiliation:
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*Research Foundation, Hospital Clínico Universitario, Valencia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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