| Progestin is an essential factor for the initiation of the meiosis in spermatogenetic cells of the eel. | |
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MedLine Citation:
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PMID: 16651536 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Meiosis is an indispensable process of sexual reproduction. However, detailed information on the regulatory mechanisms that initiate meiosis is not available. Progestins are important steroids regulating final maturation in male and female vertebrates. In male teleosts, it is known that progestin induces spermiation and sperm maturation. However, a role for progestin in early spermatogenesis or meiosis has not yet been described. In this study, we examined the functions of progestin on the initiation of meiosis in male Japanese eel. A natural progestin in teleost fish 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP) and its receptors were present in the testis at an early stage of spermatogenesis. By using an eel testicular culture system, DHP was shown to induce DNA replication in spermatogonia. Although 11-ketotestosterone, a known initiator of spermatogenesis, also stimulated DNA synthesis in spermatogonia, antibodies against DHP prevented DNA replication when added during the period in which meiosis was initiated. DHP treatment also induced the expression of meiosis-specific markers, such as DmcI and Spo11. Furthermore, Spo11 expression and synaptonemal complexes, specific features of the meiotic prophase, were detected in testicular fragments cultured with DHP in some germ cells that showed morphological characteristics of undifferentiated spermatogonia. We conclude that DHP, a progestin, is an essential factor for the initiation of meiosis. |
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Authors:
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Takeshi Miura; Masato Higuchi; Yuichi Ozaki; Takashi Ohta; Chiemi Miura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-01 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 103 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-05-10 Completed Date: 2006-07-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 7333-8 Citation Subset: IM |
Affiliation:
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Laboratory of Fish Physiology, Faculty of Agriculture, Ehime University, Matsuyama 790-8566, Japan. miutake@agr.ehime-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers Cytochromes c / genetics DNA / biosynthesis Eels / metabolism* Gene Expression / genetics Male Meiosis Organ Culture Techniques Progestins / genetics, metabolism* RNA, Messenger / genetics Receptors, Progesterone / metabolism Spermatogenesis Spermatozoa / cytology*, metabolism* Testis / metabolism Testosterone / analogs & derivatives, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Progestins; 0/RNA, Messenger; 0/Receptors, Progesterone; 53187-98-7/11-ketotestosterone; 58-22-0/Testosterone; 9007-43-6/Cytochromes c; 9007-49-2/DNA |
| Comments/Corrections | |
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