| Progesterone up-regulates WT1 mRNA and protein, and alters the relative expression of WT1 transcripts in cultured endometrial stromal cells. | |
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MedLine Citation:
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PMID: 14662165 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the change in expression of the Wilms tumor suppressor gene product, WT1, by progesterone alone in endometrial stromal cell culture and to study its relationship with prolactin, a marker of decidualization. In addition, to examine the change in ratio of WT1 isoforms with and without exon 5 message. METHODS: Endometrial biopsies were taken from eight patients who had hysterectomy. Stromal cells were isolated and cultured in the presence of progesterone alone (12 days) or progesterone and 8-bromo-cyclic adenosine monophosphate (cAMP) (6 days). RNA was extracted from cells, and reverse transcription, real-time polymerase chain reaction (PCR), and conventional PCR were done to analyze WT1 mRNA expression. Immunocytochemistry was performed on equivalent cells to study WT1 protein expression. Decidualization was identified by increased prolactin concentrations in the media and immunocytochemical markers IGFBP-1 and collagen IV. RESULTS: Reverse transcription and real-time PCR revealed a significant increase in WT1 mRNA with increasing progesterone concentrations when decidualization was occurring (n = 6, P =.002). Increasing progesterone concentrations also increased the proportion of the WT1 transcript containing a 17-amino-acid insert (+ exon 5 expression); changes in WT1 exon 5 expression have been shown to be involved in control of proliferation and differentiation. Significant correlations between WT1 message and prolactin existed at physiologic progesterone concentrations (6.25, 12.5, 25, and 50 nM; P <.05) until prolactin concentrations reached a plateau at 100 nM. At concentrations of progesterone alone (> 25 nM) and progesterone with 8-bromo-cAMP, WT1 protein was localized to the nuclei of many of the decidualized stromal cells. CONCLUSION: The changing expression of WT1 isoforms in endometrial stromal cells caused by progesterone may be important for differentiation into the decidualized phenotype. |
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Authors:
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F W Anthony; D D Mukhtar; M A Pickett; I T Cameron |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the Society for Gynecologic Investigation Volume: 10 ISSN: 1071-5576 ISO Abbreviation: J. Soc. Gynecol. Investig. Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-12-09 Completed Date: 2004-08-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9433806 Medline TA: J Soc Gynecol Investig Country: United States |
Other Details:
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Languages: eng Pagination: 509-16 Citation Subset: IM |
Affiliation:
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Maternal, Fetal and Neonatal Physiology Group, Fetal Origins of Adult Disease (FOAD) Division, University of Southampton, Princess Anne Hospital, Coxford Road, Southampton SO16 5YA, United Kingdom. fwa@soton.ac.uk |
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| MeSH Terms | |
Descriptor/Qualifier:
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8-Bromo Cyclic Adenosine Monophosphate
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pharmacology Alternative Splicing Base Sequence Cells, Cultured Collagen Type IV / metabolism Decidua / physiology Dose-Response Relationship, Drug Endometrium / cytology, drug effects, metabolism* Exons Female Gene Expression Regulation Humans Insulin-Like Growth Factor Binding Protein 1 / metabolism Molecular Sequence Data Progesterone / metabolism*, pharmacology Prolactin / metabolism RNA, Messenger / metabolism Stromal Cells / drug effects, physiology* Up-Regulation WT1 Proteins / drug effects, genetics*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type IV; 0/Insulin-Like Growth Factor Binding Protein 1; 0/RNA, Messenger; 0/WT1 Proteins; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 57-83-0/Progesterone; 9002-62-4/Prolactin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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