Document Detail

Progesterone and testosterone production by dispersed rat placental cells.
MedLine Citation:
PMID:  3697469     Owner:  NLM     Status:  MEDLINE    
Isopycnic separation and unit gravity sedimentation were employed to identify the rat placental cell types capable of producing progesterone and testosterone. Subdivision of Day 12-dispersed placental cells in Percoll gradients revealed that fractions (less than 1.048 g/ml) containing giant cytotrophoblast cells produced greater quantities of progesterone (p less than 0.01) than did fractions (greater than 1.048 g/ml) with equal numbers of placental cells but void of giant cytotrophoblasts. Unit gravity sedimentation of Day 16-dispersed placental cells revealed that when incubated, isolated giant cytotrophoblast cells were capable of producing both progesterone and testosterone. Both of the separation studies strongly suggested that other cell types also produce steroids. However, the biosynthetic capacity of the giant cytotrophoblast cell appeared to be 1000-fold greater than that of the other cell types. Incubation of Day 12-dispersed placental cells with human chorionic gonadotropin or 3',5'-cyclic adenosine monophosphate did not further increase progesterone production as compared to untreated control incubates, suggesting rat placental steroidogenesis is not under trophic hormone control. Electron microscopic observations of giant cytotrophoblast cells revealed a complex ultrastructure suggesting a variety of physiological functions.
D W Matt; J A Gibney; S Malamed; G J Macdonald
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  34     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-06-03     Completed Date:  1986-06-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  587-93     Citation Subset:  IM    
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MeSH Terms
8-Bromo Cyclic Adenosine Monophosphate / pharmacology
Cell Separation
Chorionic Gonadotropin / pharmacology
Placenta / cytology,  metabolism*
Progesterone / biosynthesis*
Testosterone / biosynthesis*
Grant Support
Reg. No./Substance:
0/Chorionic Gonadotropin; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 57-83-0/Progesterone; 58-22-0/Testosterone

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