Document Detail

Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells.
MedLine Citation:
PMID:  12672669     Owner:  NLM     Status:  MEDLINE    
Labor is preceded by cervical ripening through upregulation of interleukin (IL)-1beta, IL-8, and increased prostaglandin synthesis via inducible type 2 cyclooxygenase (COX-2). Progesterone maintains myometrial quiescence during pregnancy. In this study, we examined the effects of IL-1beta and progesterone on IL-8 and prostaglandin E2 (PGE2) synthesis and IL-8 and COX-2 mRNA and promoter activity in amnion cells and lower segment fibroblast (LSF) cells. In both cell types, progesterone had no effect on basal IL-8 or PGE2 synthesis. In LSF cells, IL-1beta significantly increased IL-8 and PGE2 synthesis and COX-2 and IL-8 mRNA expression, but progesterone significantly attenuated these effects. In prelabor amnion cells, IL-1beta also increased IL-8 and PGE2 synthesis and both COX-2 and IL-8 mRNA and promoter expression; however, progesterone significantly attenuated these effects on IL-8 and PGE2 synthesis and COX-2 expression. In postlabor amnion cells, IL-1beta increased IL-8 and PGE2 synthesis and COX-2 expression, but progesterone did not attenuate the effect of IL-1beta upon IL-8 synthesis. Progesterone repression of IL-8 and COX-2 in LSF cells suggests that IL-8 and COX-2 have similar regulatory mechanisms in LSF cells and that progesterone may play a role in maintenance of cervical competence. The lack of effect of progesterone on IL-8 in postlabor cells may be the result of downregulation of the progesterone receptor during labor.
Jenifer A Z Loudon; Catherine L Elliott; Frank Hills; Phillip R Bennett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-04-02
Journal Detail:
Title:  Biology of reproduction     Volume:  69     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-23     Completed Date:  2004-04-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  331-7     Citation Subset:  IM    
Imperial College Parturition Research Group, Wolfson and Weston Centre for Family Health, Institute of Reproductive and Developmental Biology, London W12 0HN, United Kingdom.
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MeSH Terms
Amnion / drug effects*,  enzymology,  immunology
Cells, Cultured
Cyclooxygenase 2
Dinoprostone / biosynthesis
Epithelial Cells / drug effects,  enzymology,  immunology
Fibroblasts / drug effects,  enzymology,  immunology
Interleukin-1 / pharmacology
Interleukin-8 / biosynthesis*,  genetics
Isoenzymes / genetics,  metabolism*
Labor, Obstetric / genetics,  immunology,  metabolism
Membrane Proteins
Progesterone / pharmacology*
Promoter Regions, Genetic / drug effects
Prostaglandin-Endoperoxide Synthases / genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Reg. No./Substance:
0/Interleukin-1; 0/Interleukin-8; 0/Isoenzymes; 0/Membrane Proteins; 0/RNA, Messenger; 363-24-6/Dinoprostone; 57-83-0/Progesterone; EC 2; EC protein, human; EC Synthases

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