Document Detail

Progesterone receptor localization and isoforms in myometrium, decidua, and fetal membranes from rhesus macaques: evidence for functional progesterone withdrawal at parturition.
MedLine Citation:
PMID:  12009386     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: It is not known whether withdrawal of progesterone (P) action is a prerequisite for parturition in women or in nonhuman primates because concentrations of circulating progesterone or progesterone receptors (PR) in myometrium and decidua do not decrease before delivery. To examine this potentially important regulatory mechanism, we determined PR isoforms, PR localization, and mRNA in myometrium, decidua, and fetal membranes from rhesus monkeys during pregnancy and in spontaneous labor at term. METHODS: Gestational tissues were obtained midpregnancy (day 80-100), late pregnancy (day 130-145), and during spontaneous labor at term (day 161-167). Samples of rhesus monkey myometrium, decidua, chorion-decidua, and amnion were collected and analyzed for total nuclear and cytosolic PR by competitive binding assay. Progesterone receptor isoforms were identified and quantified by Western blot analysis, and PR mRNA was determined by a specific ribonuclease protection assay. Nuclear PR was localized by immunohistochemistry with monoclonal anti-PR (JZB39) after microwave stabilization. RESULTS: Myometrium and decidua showed no change in total PR during pregnancy and labor. Nuclear PR was not detected in fetal membranes by binding assay but was localized in amnion epithelial and mesenchymal cells and in chorion laeve cytotrophoblasts by immunohistochemistry. Staining for PR was substantially less by serial antibody dilution in fetal membranes than in decidua. Message for PR was confirmed in all tissues analyzed. A significant (P <.05) shift in the ratio of PR isoforms (from PR-B dominance at midpregnancy to PR-A dominance in labor) was observed in myometrium but not in decidua. Both PR-A and PR-B isoforms and PR nuclear staining were nearly undetectable in amnion obtained during labor. CONCLUSION: A shift to PR-A dominance in myometrium at term together with a loss of PR in fetal membranes provides evidence for a functional progesterone withdrawal mechanism, which may facilitate the initiation of parturition in primates.
G J Haluska; T R Wells; J J Hirst; R M Brenner; D W Sadowsky; M J Novy
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the Society for Gynecologic Investigation     Volume:  9     ISSN:  1071-5576     ISO Abbreviation:  J. Soc. Gynecol. Investig.     Publication Date:    2002 May-Jun
Date Detail:
Created Date:  2002-05-15     Completed Date:  2002-09-16     Revised Date:  2010-03-23    
Medline Journal Info:
Nlm Unique ID:  9433806     Medline TA:  J Soc Gynecol Investig     Country:  United States    
Other Details:
Languages:  eng     Pagination:  125-36     Citation Subset:  IM    
Division of Reproductive Sciences, Oregon Regional Primate Research Center/Oregon Health & Science University, 505 NW 185th Avenue, Beaverton, OR 97006-3499, USA.
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MeSH Terms
Decidua / cytology*
Extraembryonic Membranes / cytology*
Labor, Obstetric / physiology*
Macaca mulatta
Myometrium / cytology*
Pregnancy, Animal / physiology*
Progesterone / physiology*
Protein Isoforms / analysis
Receptors, Progesterone / analysis*,  chemistry
Grant Support
Reg. No./Substance:
0/Protein Isoforms; 0/Receptors, Progesterone; 57-83-0/Progesterone
Comment In:
J Soc Gynecol Investig. 2002 May-Jun;9(3):117   [PMID:  12009384 ]

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