Document Detail


Progesterone receptor concentration differences in the chick oviduct cells and apparent down-regulation by ligand. A semiquantitative immunohistochemical study.
MedLine Citation:
PMID:  2696849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A semiquantitative immunohistochemical technique was developed for identification of chick progesterone receptor (PR). The mouse monoclonal antibody PR6 was used. The nuclear PR concentration was analyzed with Leitz Orthoplan MPV-3 light microscope. The target tissue was chick oviduct, with epithelial, glandular, mesenchymal, smooth muscle and peritoneal cells analyzed separately. PR concentration varied between different cell types and also from cell to cell within a single cell type. A significant decrease of PR concentration, as noted by decrease in staining, was also observed in all studied cell types, 6 h after a single injection of progesterone. This technique allows for histological identification of biochemical events that should help lead to the understanding of the role of PR changes in a variety of experimental situations.
Authors:
A Pekki; T Joensuu; K Vidqvist; D Toft; P Tuohimaa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of steroid biochemistry     Volume:  34     ISSN:  0022-4731     ISO Abbreviation:  J. Steroid Biochem.     Publication Date:  1989  
Date Detail:
Created Date:  1990-03-30     Completed Date:  1990-03-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0260125     Medline TA:  J Steroid Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  351-4     Citation Subset:  IM    
Affiliation:
Department of Biomedical Sciences, University of Tampere, Finland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / diagnostic use
Chickens
Down-Regulation*
Female
Immunoenzyme Techniques
Ligands
Oviducts / cytology,  drug effects,  metabolism*
Progesterone / pharmacology*
Receptors, Progesterone / drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Ligands; 0/Receptors, Progesterone; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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