Document Detail

Progesterone promotes the acrosome reaction in capacitated human spermatozoa as judged by flow cytometry and CD46 staining.
MedLine Citation:
PMID:  10340996     Owner:  NLM     Status:  MEDLINE    
The acrosome reaction is a necessary prerequisite for spermatozoa to acquire fertilizing ability. Several different moieties appear to promote the acrosome reaction through different pathways, including solubilized zona pellucidae, recombinant zona protein ZP3, follicular fluid, calcium ionophores, and mannosylated bovine serum albumin (BSA). Although many investigators have presented evidence that progesterone also promotes the acrosome reaction through the mediation of a non-genomic cell membrane receptor, this concept has been challenged. Other workers have suggested that progesterone does not promote an acrosome reaction in human spermatozoa, as judged by the detection of CD46, a complement regulatory protein present on the inner acrosome membrane, through flow cytometric analysis of large numbers of spermatozoa. Prior investigations were criticized by the limited numbers of spermatozoa enumerated visually, the use of non-specific staining techniques, and the failure to eliminate dead spermatozoa during the scoring of the acrosome reaction. We have repeated these experiments, using both a supravital dye to eliminate dead spermatozoa from flow cytometric analysis, and anti-CD46 monoclonal antibody to score acrosome-reacted spermatozoa. Care was taken to validate the adequacy of capacitation conditions, which were proven by the ability of spermatozoa to acrosome react in response to mannosylated BSA and to penetrate zona-free hamster eggs. Confocal microscopy was used to confirm that CD46 immunostaining was limited to the acrosomal region of the spermatozoon head. Our results indicate that progesterone does promote an acrosome reaction within capacitated spermatozoa.
R A Bronson; T Peresleni; M Golightly
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Molecular human reproduction     Volume:  5     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-07-20     Completed Date:  1999-07-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  507-12     Citation Subset:  IM    
Department of Obstetrics & Gynecology, Health Sciences Center, State University of New York at Stony Brook, 11794-8091, USA.
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MeSH Terms
Acrosome Reaction / drug effects,  physiology*
Antigens, CD / analysis,  metabolism*
Antigens, CD46
Bisbenzimidazole / chemistry
Cell Death
Flow Cytometry / methods
Infertility, Male
Mannose / pharmacology
Membrane Glycoproteins / analysis,  metabolism*
Microscopy, Confocal
Progesterone / pharmacology,  physiology*
Serum Albumin / pharmacology
Sperm-Ovum Interactions
Spermatozoa / drug effects,  physiology*
Staining and Labeling / methods
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD46; 0/CD46 protein, human; 0/Membrane Glycoproteins; 0/Serum Albumin; 0/mannose-bovine serum albumin conjugate; 23491-44-3/Bisbenzimidazole; 31103-86-3/Mannose; 57-83-0/Progesterone

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