Document Detail

Progesterone exposure prevents matrix metalloproteinase-3 (MMP-3) stimulation by interleukin-1alpha in human endometrial stromal cells.
MedLine Citation:
PMID:  10770206     Owner:  NLM     Status:  MEDLINE    
Suppression of endometrial matrix metalloproteinases (MMPs) is necessary to maintain tissue stability during the invasive events of implantation and placental development. Several laboratories have shown that inflammatory cytokines, including interleukin-lalpha (IL-1alpha), can oppose progesterone suppression of MMPs in the human endometrium. Furthermore, we have recently demonstrated colocalization of epithelial cell IL-1alpha and MMP-7 expression at sites of ectopic pregnancy. The current study extends these findings, revealing a previously unrecognized interrelationship between progesterone and IL-1alpha in regulation of MMP-3. Although IL-1alpha is a potent stimulator of MMP-3 in proliferative phase endometrium in organ culture, we demonstrate that progesterone exposure in vivo reduces IL-1alpha stimulation of MMP-3 in secretory phase tissue. This loss of sensitivity to IL-1alpha was duplicated in isolated stromal cells treated with progesterone in vitro, and IL-1alpha stimulation of MMP-3 returned in a dose-dependent manner with progesterone withdrawal. The antiprogestin, onapristone, partially blocked the ability of progesterone to prevent stimulation of MMP-3 by IL-1alpha. These data suggest a novel mechanism by which progesterone may preserve tissue integrity during the establishment and maintenance of pregnancy by limiting stimulation of MMPs by inflammatory cytokines such as IL-1a.
N R Keller; E Sierra-Rivera; E Eisenberg; K G Osteen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  85     ISSN:  0021-972X     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-01     Completed Date:  2000-05-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1611-9     Citation Subset:  AIM; IM    
Department of Cellular and Molecular Pathology and the Women's Reproductive Health Research Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
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MeSH Terms
Endometrium / enzymology*
Gonanes / pharmacology
Hormone Antagonists / pharmacology
Interleukin-1 / pharmacology*
Matrix Metalloproteinase 3 / metabolism*
Menstrual Cycle
Organ Culture Techniques
Progesterone / administration & dosage,  antagonists & inhibitors,  pharmacology*
Stromal Cells / enzymology*
Grant Support
Reg. No./Substance:
0/Gonanes; 0/Hormone Antagonists; 0/Interleukin-1; 57-83-0/Progesterone; 96346-61-1/onapristone; EC Metalloproteinase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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