Document Detail


Progesterone analogs influence germination of Clostridium sordellii and Clostridium difficile spores in vitro.
MedLine Citation:
PMID:  21478359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clostridium sordellii and Clostridium difficile are closely related anaerobic Gram-positive, spore-forming human pathogens. C. sordellii and C. difficile form spores that are believed to be the infectious form of these bacteria. These spores return to toxin-producing vegetative cells upon binding to small molecule germinants. The endogenous compounds that regulate clostridial spore germination are not fully understood. While C. sordellii spores require three structurally distinct amino acids to germinate, the occurrence of postpregnancy C. sordellii infections suggests that steroidal sex hormones might regulate its capacity to germinate. On the other hand, C. difficile spores require taurocholate (a bile salt) and glycine (an amino acid) to germinate. Bile salts and steroid hormones are biosynthesized from cholesterol, suggesting that the common sterane structure can affect the germination of both C. sordellii and C. difficile spores. Therefore, we tested the effect of sterane compounds on C. sordellii and C. difficile spore germination. Our results show that both steroid hormones and bile salts are able to increase C. sordellii spore germination rates. In contrast, a subset of steroid hormones acted as competitive inhibitors of C. difficile spore germination. Thus, even though C. sordellii and C. difficile are phylogenetically related, the two species' spores respond differently to steroidal compounds.
Authors:
Marc Liggins; Norma Ramirez; Natiera Magnuson; Ernesto Abel-Santos
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Publication Detail:
Type:  Journal Article     Date:  2011-04-08
Journal Detail:
Title:  Journal of bacteriology     Volume:  193     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-13     Completed Date:  2011-07-13     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2776-83     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Nevada, Las Vegas, Las Vegas, Nevada 89154, USA.
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MeSH Terms
Descriptor/Qualifier:
Bile Acids and Salts / metabolism*
Clostridium difficile / growth & development*,  metabolism
Clostridium sordellii / growth & development*,  metabolism
Humans
Progesterone / analogs & derivatives*,  metabolism*
Spores, Bacterial / growth & development*,  metabolism
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 57-83-0/Progesterone
Comments/Corrections

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