Document Detail


Progesterone modulates the proliferation and differentiation of human periodontal ligament cells.
MedLine Citation:
PMID:  20532879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hormone deficiency has been recognized as a risk factor for periodontal disease in postmenopausal women. However, the anabolic effects of progesterone on human periodontal ligament cells (hPDLCs) are still unclear. Therefore, the objective of this study was to detect the expression of progesterone receptor (PgR) in hPDLCs and investigate the bone-sparing effects of progesterone. We detected PgR expression in hPDLCs by reverse transcriptase-polymerase chain reaction and immunocytochemistry. After progesterone stimulation, the percentage of hPDLCs entering the S + G2M phase of the cell cycle increased significantly, accompanied by an increased cell growth curve. In both basic culture medium and osteogenic medium, progesterone activated alkaline phosphatase-positive cells and alizarin red-positive nodules. Moreover, mineralization-related markers were up-regulated by progesterone in both time-dependent and dose-dependent manners. In contrast, these effects of progesterone were blocked by the PgR antagonist (RU486). Our results demonstrated that the PgR is expressed in hPDLCs at the gene and protein level, and that progesterone can stimulate the proliferation and differentiation of the hPDLCs. These findings suggest that progesterone may play a significant role in osteoblastic function of hPDLCs and may influence the maintenance of alveolar bone mass.
Authors:
Gongjie Yuan; Chuan Cai; Juan Dai; Yali Liu; Rui Zhang; Yuanyuan Dai; Li Wen; Yin Ding
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-09
Journal Detail:
Title:  Calcified tissue international     Volume:  87     ISSN:  1432-0827     ISO Abbreviation:  Calcif. Tissue Int.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-14     Completed Date:  2010-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905481     Medline TA:  Calcif Tissue Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  158-67     Citation Subset:  IM    
Affiliation:
Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Alkaline Phosphatase / biosynthesis,  genetics,  metabolism
Cell Differentiation / drug effects*,  genetics
Cell Proliferation / drug effects
Cells, Cultured
Culture Media / metabolism,  pharmacology
Female
Humans
Periodontal Ligament / cytology*,  drug effects,  metabolism
Progesterone / genetics,  metabolism,  pharmacology*
Receptors, Progesterone / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation
Young Adult
Chemical
Reg. No./Substance:
0/Culture Media; 0/Receptors, Progesterone; 57-83-0/Progesterone; EC 3.1.3.1/Alkaline Phosphatase

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