Document Detail


Progenitor cells harvested from bovine follicles become endothelial cells.
MedLine Citation:
PMID:  20303645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hematopoietic-like colonies develop in post-confluent granulosa cell cultures derived from bovine antral follicles. Previously, we had shown that these colonies gave rise to macrophages. In the present study, we validated the presence of somatic KIT-positive (KIT(+)) progenitor cells in colony-containing granulosa cell cultures. The cultures expressed the progenitor cell markers Sox-2, Oct 3/4, KIT, and alkaline phosphatase in western blot analysis. The successful double immunofluorescence localization of KIT and CD14, CD45, CD133, or VEGF-R2 revealed a specific subpopulation of progenitor cells. Flow cytometry showed that cells doubly positive for KIT and CD14 or CD45 comprised less than 10% of the population. The KIT(+) cells were purified by magnetic selection and differentiated with the hanging drop technique using haematopoietic differentiation medium. Pure cultures of either granulosa cells or endothelial cells were obtained. The spindle-shaped and epithelioid phenotypes indicated endothelial cell heterogeneity of microvascular source. We conclude that progenitor cells are obtained from the follicle harvest, which differentiate into endothelial cells. The cells are relevant for findings to angiogenesis and luteinization of the corpus luteum.
Authors:
Claudia Merkwitz; Albert M Ricken; Andreas Lösche; Michiharu Sakurai; Katharina Spanel-Borowski
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Publication Detail:
Type:  Journal Article     Date:  2010-03-19
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  79     ISSN:  1432-0436     ISO Abbreviation:  Differentiation     Publication Date:    2010 Apr-Jun
Date Detail:
Created Date:  2010-05-17     Completed Date:  2010-08-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  England    
Other Details:
Languages:  eng     Pagination:  203-10     Citation Subset:  IM    
Affiliation:
Institute of Anatomy, University of Leipzig, Liebigstrasse 13, D-04103 Leipzig, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / metabolism
Biological Markers / metabolism
Cattle
Cell Differentiation / physiology*
Cell Separation / methods
Cells, Cultured
Endothelial Cells / cytology,  physiology*
Female
Granulosa Cells / cytology,  metabolism
Ovarian Follicle / cytology*
Proto-Oncogene Proteins c-kit / metabolism
Stem Cells / cytology,  physiology*
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Biological Markers; EC 2.7.10.1/Proto-Oncogene Proteins c-kit

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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