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Progenitor cell homing in the postischemic myocardium: just an unmotivated pitstop in the microcirculation ?
MedLine Citation:
PMID:  22827532     Owner:  NLM     Status:  Publisher    
OBJECTIVE: We developed a model for direct assessment of bone marrow-derived progenitor cell (BMC) sequestration in the postischemic murine myocardium after direct antegrade intracoronary injection. METHODS: Modified syngeneic heterotopic heart transplantation was used as a basic model for global myocardial ischemia/reperfusion (I/R) injury in a total of n=29 animals. Intravital fluorescence microscopy (IVM) was employed to analyze of the right ventricular subepicardial coronary microcirculation and for tracking fluorescently labeled BMCs. RESULTS: IVM allowed to monitor all segments of the coronary microcirculation including feeding arterioles, nutritive capillaries and postcapillary venules. Warm ischemia and generalized atherosclerosis induced profound reperfusion failure, particularly in nutritive myocardial capillaries. BMCs were found to exclusively sequester in myocardial capillaries, but not in coronary arterioles or postcapillary venules. The sequestration of BMCs in coronary capillaries occurred independent of warm ischemia, generalized atherosclerosis or adhesion molecule function. CONCLUSIONS: This is the first study allowing direct assessment of BMC homing to the postischemic myocardium. Heterotopic heart transplantation and intravital fluorescence microscopy are proper means to study the myocardial sequestration of BMCs after direct antegrade intracoronary injection in vivo. We show for the first time that intracoronarily injected BMCs sequester exclusively in nutritive myocardial capillaries. © 2012 John Wiley & Sons Ltd.
Fabio Tuche; Michael D Menger; Christina Körbel; Ruth M Nickels; Eliete Bouskela; René Schramm
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-25
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  -     ISSN:  1549-8719     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 John Wiley & Sons Ltd.
Institute for Clinical and Experimental Surgery, University of Saarland, Medical Faculty, Kirrbergerstrasse, D-66421, Homburg/Saar, Germany; Laboratory for Clinical and Experimental Research in Vascular Biology - BioVasc - Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
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