Document Detail


Profiling resistance-related mutations in the protease region of the pol gene: single genome sequencing of HIV in plasma and peripheral blood mononuclear cells.
MedLine Citation:
PMID:  18593345     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genotypic resistance is currently assessed through direct sequencing, which cannot detect resistant strains below 20%. We compared the genotypic resistance profile of virions and proviruses using population-based analysis and single genome sequencing of the protease region of the pol gene in samples collected from five individuals in whom indinavir monotherapy resulted in treatment failure. Single genome sequencing showed that not all strains present the same resistance mutations, which can be dispersed across different HIV genomes. The resistance profile found in plasma was very similar to that found in peripheral blood mononuclear cells (PBMCs), confirming the utility of assessing proviral DNA as for a means of determining antiretroviral resistance.
Authors:
Ana Paula Guimarães; Dercy Jose Sa-Filho; Maria Cecilia Sucupira; Luiz Mario Janini; Ricardo Sobhie Diaz
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  AIDS research and human retroviruses     Volume:  24     ISSN:  1931-8405     ISO Abbreviation:  AIDS Res. Hum. Retroviruses     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-08-01     Completed Date:  2008-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709376     Medline TA:  AIDS Res Hum Retroviruses     Country:  United States    
Other Details:
Languages:  eng     Pagination:  969-71     Citation Subset:  IM; X    
Affiliation:
Federal University of São Paulo, São Paulo, Brazil 04020-041.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Drug Resistance, Viral / genetics
HIV / genetics*
HIV Infections / drug therapy*,  virology*
HIV Protease / genetics*
HIV Protease Inhibitors / pharmacology*,  therapeutic use*
Humans
Indinavir / pharmacology*,  therapeutic use*
Leukocytes, Mononuclear / virology*
Molecular Sequence Data
Mutation
Plasma / virology*
Proviruses / genetics
Sequence Alignment
Treatment Failure
Virion / genetics
Chemical
Reg. No./Substance:
0/HIV Protease Inhibitors; 150378-17-9/Indinavir; EC 3.4.23.-/HIV Protease

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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