| Profiling the proteome dynamics during the cell cycle of human hepatoma cells. | |
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MedLine Citation:
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PMID: 18655025 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Deregulation of cell cycle leads to cell transformation and cancer development. Here we present profiling the proteome dynamics using 2-DE and constructing the associated functional networks during the cell cycle of human hepatoma cells, Mahlavu. The protein dynamics was validated by hierarchical clustering analysis on the proteome, and by Northern blot assays on the selected 14-3-3 proteins. Of the 2665 protein spots, 201 with variation coefficient of expression dynamics >20% throughout the cell cycle were subjected to analysis. Degree of the global protein dynamics was phase dependent with the greatest in transitional phases of S/G2, G2/M, and G1/S. Concurrence of pathways coordinating cell-cycle progression versus arrest, and/or pathways regulating apoptosis versus antiapoptosis was always identified during the cell cycle, suggesting the existence of counteracting mechanisms for intracellular homeostasis. Data mining of the results suggested that the key transcription factors in G0/G1, G1/S, S, and G2/M were p53 and SP1, c-Myc, c-Myc and p53, and YY1 and c-Jun, respectively. Our findings for the first time provide insights into the regulation of mammalian cell-cycle progression at the proteome level, and grant a model to study disease mechanisms and to discover therapeutic targets for anticancer therapy. |
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Authors:
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Sen-Yung Hsieh; Feng-Hui Zhuang; Yi-Ting Wu; Jen-Kun Chen; Yun-Lin Lee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proteomics Volume: 8 ISSN: 1615-9861 ISO Abbreviation: Proteomics Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-29 Completed Date: 2008-09-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 2872-84 Citation Subset: IM |
Affiliation:
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Clinical Proteomics Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan. siming.shia@msa.hinet.net |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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chemistry,
metabolism*,
pathology Cell Cycle / physiology* Cell Death / genetics, physiology Cell Line, Tumor Cluster Analysis Cytoskeleton / chemistry, genetics, physiology Flow Cytometry Gene Expression Regulation, Neoplastic* Humans Liver Neoplasms / chemistry, metabolism*, pathology Neoplasm Proteins / biosynthesis, genetics, physiology Protein Isoforms / biosynthesis, genetics, physiology Proteome / biosynthesis*, genetics, metabolism Signal Transduction / genetics, physiology |
| Chemical | |
Reg. No./Substance:
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0/Neoplasm Proteins; 0/Protein Isoforms; 0/Proteome |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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