Document Detail

Profiling pluripotent stem cells and organelles using synchrotron radiation infrared microspectroscopy.
MedLine Citation:
PMID:  23125135     Owner:  NLM     Status:  Publisher    
FTIR micro-spectroscopy is a sensitive, non-destructive and label-free method offering diffraction-limited resolution with high signal-to-noise ratios when combined with a synchrotron radiation source. The vibrational signature of individual cells was used to validate an alternative strategy for reprogramming induced pluripotent stem cells generated from amniocytes. The iPSC lines PB09 and PB10, were reprogrammed from the same amniocyte cell line using respectively the Oct54, Sox2, Lin28, and Nanog and the Oct4 and Sox2 transcription factor cocktail. We show that cells reprogrammed by the two different sets of transfection factors have similar spectral signatures after reprogramming, except for a small subpopulation of cells in one of the cell lines. Mapping HeLa cells at subcellular resolution, we show that the Golgi apparatus, the cytoplasm and the nucleus have a specific spectral signature. The CH(3) :CH(2) ratio is the highest in the nucleus and the lowest in the Golgi apparatus/endoplasmic reticulum, in agreement with the membrane composition of these organelles. This is confirmed by specific staining of the organelles with fluorescent dyes. Subcellular differentiation of cell compartments is also demonstrated in living cells. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
Christophe Sandt; Joni Frederick; Paul Dumas
Related Documents :
24114775 - Endothelin-1 receptor a blocker darusentan decreased hepatic changes and improved liver...
24014685 - Mesenchymal stromal cell turnover in the normal adult lung revisited.
23649725 - Enac contribution to epithelial wound healing is independent of the healing mode and of...
24336085 - Induction of necrotic cell death by oxidative stress in retinal pigment epithelial cells.
25219685 - Characterization and expression of proprotein convertases in cho cells: efficient prote...
19075245 - Paneth cells directly sense gut commensals and maintain homeostasis at the intestinal h...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-2
Journal Detail:
Title:  Journal of biophotonics     Volume:  -     ISSN:  1864-0648     ISO Abbreviation:  J Biophotonics     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101318567     Medline TA:  J Biophotonics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Synchrotron SOLEIL, L'Orme des Merisiers, BP48, 91192, Gif-sur-Yvette, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Exposure of pregnant mice to chlorpyrifos-methyl alters embryonic H19 gene methylation patterns.
Next Document:  Calcium-phosphate microprecipitates mimic microparticles when examined with flow cytometry.