Document Detail


Profiling metabolites and peptides in single cells.
MedLine Citation:
PMID:  21451513     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intracellular levels and spatial localizations of metabolites and peptides reflect the state of a cell and its relationship to its surrounding environment. Moreover, the amounts and dynamics of metabolites and peptides are indicative of normal or pathological cellular conditions. Here we highlight established and evolving strategies for characterizing the metabolome and peptidome of single cells. Focused studies of the chemical composition of individual cells and functionally defined groups of cells promise to provide a greater understanding of cell fate, function and homeostatic balance. Single-cell bioanalytical microanalysis has also become increasingly valuable for examining cellular heterogeneity, particularly in the fields of neuroscience, stem cell biology and developmental biology.
Authors:
Stanislav S Rubakhin; Elena V Romanova; Peter Nemes; Jonathan V Sweedler
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Review     Date:  2011-03-30
Journal Detail:
Title:  Nature methods     Volume:  8     ISSN:  1548-7105     ISO Abbreviation:  Nat. Methods     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-31     Completed Date:  2011-05-06     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101215604     Medline TA:  Nat Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  S20-9     Citation Subset:  IM    
Affiliation:
Department of Chemistry and the Beckman Institute, University of Illinois at Urbana-Champaign, Urbana-Champaign, Illinois, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation
Chromatography
Data Interpretation, Statistical
Electrophoresis
Humans
Magnetic Resonance Spectroscopy
Mass Spectrometry
Metabolome
Metabolomics / methods*,  statistics & numerical data
Microfluidic Analytical Techniques
Proteome
Proteomics / methods*,  statistics & numerical data
Single-Cell Analysis / methods*,  statistics & numerical data,  trends
Grant Support
ID/Acronym/Agency:
5R01DE018866/DE/NIDCR NIH HHS; 5R01NS031609/NS/NINDS NIH HHS; P30 DA018310/DA/NIDA NIH HHS; P30 DA018310-08/DA/NIDA NIH HHS; R01 DE018866-04/DE/NIDCR NIH HHS; R01 DE018866-05/DE/NIDCR NIH HHS; R01 NS031609-15/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Proteome
Comments/Corrections

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