Document Detail


Profiling of extracellular matrix and cadherin family gene expressions in mouse feeder layer cells: Type VI collagen is a candidate molecule inducing the colony formation of epithelial cells.
MedLine Citation:
PMID:  22784000     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Mouse 3T3 feeder layer has been utilized for epidermal and corneal epithelial cell culture to promote tissue-like cell stratification. However, the molecular mechanism underlying epithelial-feeder layer interactions remains poorly understood. Here, the feeder layer activity of six different mouse cell lines were examined in terms of the colony forming efficiency of primary limbal epithelial cells including corneal epithelial stem/progenitor cells. When epithelial cells and feeder layers were separated by culture inserts, the colony forming efficiency was significantly lower than that of epithelial cells, which were cultured with feeder cells on the same dish surfaces, implying that direct contacts between these cells and/or pericellular extracellular matrix (ECM) deposition by feeder layers have an important role in feeder layer activity. With TaqMan PCR assay, the gene expression of 29 ECM molecules and 32 cadherin family genes were profiled in two highest and two lowest cell lines in colony forming efficiency for limbal and oral mucosal epithelial cells. Significant difference in the expression correlated with colony forming efficiency was observed in six ECM molecules and four kinds of cadherin family genes. In these results, type VI collagen was confirmed to be able to promote the colony formation of epithelial cells in vitro effectively.
Authors:
Ryo Takagi; Masayuki Yamato; Ai Kushida; Kohji Nishida; Teruo Okano
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-11
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Tokyo Women's Medical University, Advanced Biomaterial Engineering and Science, 8-1 Kawada-Cho, Shinjuku-ku, Tokyo, Tokyo, Japan, 162-8666, +81-3353-8112; rtakagi@abmes.twmu.ac.jp.
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