Document Detail


Profiling Ubiquitin Linkage Specificities of Deubiquitinating Enzymes with Branched Ubiquitin Isopeptide Probes.
MedLine Citation:
PMID:  22689415     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Branching out: Novel branched ubiquitin isopeptide activity-based probes (UIPPs) were engineered to profile ubiquitin (Ub) linkage specificity of deubiquitinating enzymes (DUBs) in cell extracts. Using chemical proteomics, a preference towards K48-linked Ub was shown for USP5, USP7, UCH L-3, and UCH L-5, whereas USP19 and USP38 preferred K63-linked Ub.
Authors:
Alexander Iphöfer; Anne Kummer; Manfred Nimtz; Antje Ritter; Tatjana Arnold; Ronald Frank; Joop van den Heuvel; Benedikt M Kessler; Lothar Jänsch; Raimo Franke
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-11
Journal Detail:
Title:  Chembiochem : a European journal of chemical biology     Volume:  -     ISSN:  1439-7633     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100937360     Medline TA:  Chembiochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Department of Molecular Structural Biology, Cellular Proteomics, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig (Germany).
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