Document Detail


Profiling TRA-1-81 antigen distribution on a human embryonic stem cell.
MedLine Citation:
PMID:  18313397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human embryonic stem (hES) cells hold great promise in regenerative medicine. Although hES cells have unlimited self-renewal potential, they tend to differentiate spontaneously in culture. TRA-1-81 is a biomarker of undifferentiated hES cells. Quantitative characterization of TRA-1-81 expression level in a single cell helps capture the "turn-on" signal and understand the mechanism of early differentiation. Here, we report on our examination of TRA-1-81 distribution and association on a hES cell membrane using an atomic force microscope (AFM). Our results suggest that aggregated distribution of TRA-1-81 antigen is characteristic for undifferentiated hES cells. We also evaluated the TRA-1-81 expression level at approximately 17,800 epitopes and approximately 700 epitopes per cell on an undifferentiated cell and a spontaneously differentiated cell, respectively. The method in this study can be adapted in examining other surface proteins on various cell types, thus providing a general tool for investigating protein distribution and association at the single cell level.
Authors:
Dengli Qiu; Jialing Xiang; Zhaoxia Li; Aparna Krishnamoorthy; Liaohai Chen; Rong Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-02-29
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  369     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-03-25     Completed Date:  2008-04-22     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  735-40     Citation Subset:  IM    
Affiliation:
Department of Biological, Chemical and Physical Sciences, Illinois Institute of Technology, 3101 S. Dearborn Street, Life Sciences Building, Chicago, IL 60616, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Surface / immunology*
Cell Line
Cell Membrane / immunology*
Embryonic Stem Cells / immunology*
Epitope Mapping / methods*
Humans
Grant Support
ID/Acronym/Agency:
R01 NS047719/NS/NINDS NIH HHS; R01 NS047719-01A1/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Surface; 0/TRA-1-81 antigen, human
Comments/Corrections

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