Document Detail


Profiling of fatty acids released during calcium-induced mitochondrial permeability transition in isolated rabbit kidney cortex mitochondria.
MedLine Citation:
PMID:  21443943     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increases in intracellular Ca(2+) during cellular stress often lead to the mitochondrial permeability transition (MPT). We examined changes in fatty acids (FAs) released from isolated renal cortical mitochondria subjected to Ca(2+)-induced MPT. Exposing mitochondria to Ca(2+) stimulated mitochondrial swelling and release of FAs such as arachidonic (20:4) and docosahexenoic acids which increased 71% and 32%, respectively, and linoleic (18:2) which decreased 23% compared to controls. Stearic (18:0), oleic (18:1), and linoleic (18:3) acids were unchanged. To elucidate a mechanism for FA release, mitochondria were pre-treated with bromoenolactone (BEL) to inhibit Ca(2+)-independent phospholipase A(2) gamma activity (iPLA(2)γ). BEL blocked Ca(2+)-induced release of arachidonic and behenic (22:0) acids. Finally, four FAs were released in the absence of Ca(2+) in a BEL-sensitive manner, including arachidonic and docosatrienoic acids. Thus, extensive FA release occurs during Ca(2+)-induced MPT, and that mitochondrial iPLA(2)γ maintains mitochondrial arachidonic acid homeostasis under both basal and Ca(2+)-induced stress conditions.
Authors:
Jason L Blum; Gilbert R Kinsey; Prashant Monian; Bin Sun; Brian S Cummings; Jane McHowat; Rick G Schnellmann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-04-03
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  25     ISSN:  1879-3177     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-06-06     Completed Date:  2011-09-26     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  1001-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Center for Cell Death, Injury, and Regeneration, Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC 29425, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid / secretion
Calcium / analysis,  metabolism*
Docosahexaenoic Acids / secretion
Fatty Acids / metabolism*
Female
Group VI Phospholipases A2 / antagonists & inhibitors
Homeostasis / drug effects
Kidney Cortex / drug effects*
Linoleic Acid / secretion
Mitochondria / drug effects*
Mitochondrial Membrane Transport Proteins / metabolism
Mitochondrial Swelling / drug effects
Models, Animal
Permeability
Rabbits
Grant Support
ID/Acronym/Agency:
C06 RR-015455/RR/NCRR NIH HHS; DK-62028/DK/NIDDK NIH HHS; F32 DK-081267/DK/NIDDK NIH HHS; P20 RR-016461/RR/NCRR NIH HHS; R01 DK062028-07/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Mitochondrial Membrane Transport Proteins; 2197-37-7/Linoleic Acid; 25167-62-8/Docosahexaenoic Acids; 506-32-1/Arachidonic Acid; 7440-70-2/Calcium; EC 3.1.1.4/Group VI Phospholipases A2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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