| Profiling of fatty acids released during calcium-induced mitochondrial permeability transition in isolated rabbit kidney cortex mitochondria. | |
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MedLine Citation:
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PMID: 21443943 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Increases in intracellular Ca(2+) during cellular stress often lead to the mitochondrial permeability transition (MPT). We examined changes in fatty acids (FAs) released from isolated renal cortical mitochondria subjected to Ca(2+)-induced MPT. Exposing mitochondria to Ca(2+) stimulated mitochondrial swelling and release of FAs such as arachidonic (20:4) and docosahexenoic acids which increased 71% and 32%, respectively, and linoleic (18:2) which decreased 23% compared to controls. Stearic (18:0), oleic (18:1), and linoleic (18:3) acids were unchanged. To elucidate a mechanism for FA release, mitochondria were pre-treated with bromoenolactone (BEL) to inhibit Ca(2+)-independent phospholipase A(2) gamma activity (iPLA(2)γ). BEL blocked Ca(2+)-induced release of arachidonic and behenic (22:0) acids. Finally, four FAs were released in the absence of Ca(2+) in a BEL-sensitive manner, including arachidonic and docosatrienoic acids. Thus, extensive FA release occurs during Ca(2+)-induced MPT, and that mitochondrial iPLA(2)γ maintains mitochondrial arachidonic acid homeostasis under both basal and Ca(2+)-induced stress conditions. |
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Authors:
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Jason L Blum; Gilbert R Kinsey; Prashant Monian; Bin Sun; Brian S Cummings; Jane McHowat; Rick G Schnellmann |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-04-03 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 25 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-06-06 Completed Date: 2011-09-26 Revised Date: 2013-05-24 |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 1001-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Center for Cell Death, Injury, and Regeneration, Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC 29425, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / secretion Calcium / analysis, metabolism* Docosahexaenoic Acids / secretion Fatty Acids / metabolism* Female Group VI Phospholipases A2 / antagonists & inhibitors Homeostasis / drug effects Kidney Cortex / drug effects* Linoleic Acid / secretion Mitochondria / drug effects* Mitochondrial Membrane Transport Proteins / metabolism Mitochondrial Swelling / drug effects Models, Animal Permeability Rabbits |
| Grant Support | |
ID/Acronym/Agency:
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C06 RR-015455/RR/NCRR NIH HHS; DK-62028/DK/NIDDK NIH HHS; F32 DK-081267/DK/NIDDK NIH HHS; P20 RR-016461/RR/NCRR NIH HHS; R01 DK062028-07/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Mitochondrial Membrane Transport Proteins; 2197-37-7/Linoleic Acid; 25167-62-8/Docosahexaenoic Acids; 506-32-1/Arachidonic Acid; 7440-70-2/Calcium; EC 3.1.1.4/Group VI Phospholipases A2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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