Document Detail

Profiles of cell-to-cell interaction of Mycobacterium intracellulare-induced immunosuppressive macrophages with target T cells in terms of suppressor signal transmission.
MedLine Citation:
PMID:  12165083     Owner:  NLM     Status:  MEDLINE    
Previously, we have found that immunosuppressive macrophages (M(phi)s) induced by Mycobacterium intracellulare-infection (MI-M(phi)s) required cell contact with target T cells to express their suppressor activity against concanavalin A (Con A)-induced T cell mitogenesis. In this study, we examined the profiles of cell-to-cell interaction of MI-M(phi)s with target T cells. First, MI-M(phi)s displayed suppressor activity in an H-2 allele-unrestricted manner, indicating that MHC molecules are not required for cell contact. The suppressor activity of MI-M(phi)s was reduced markedly by paraformaldehyde fixation or treatment with cytochalasin B or colchicine, indicating that vital membrane functions are required for their suppressor activity. Secondly, the suppressor activity of MI-M(phi)s was independent of cell-to-cell interaction via CD40 ligand/CD40 and M(phi)-derived indoleamine 2,3-dioxygenase, which causes rapid degradation of tryptophan in T cells. Thirdly, precultivation of splenocytes with MI-M(phi)s, allowing cell-to-cell contact, reduced Con A- or anti-CD3 antibody-induced mitogenesis but not phorbol myristate acetate/calcium ionophore A23187-elicited proliferation of T cells. In addition, co-cultivation of T cells with MI-M(phi)s caused marked changes in profiles of the tyrosine phosphorylation of 33 kDa, 34 kDa and 35-kDa proteins and, moreover, the activation of protein kinase C and its translocation to the cell membrane. It thus appears that suppressor signals of MI-M(phi)s, which are transmitted to the target T cells via cell contact, principally cross-talk with the early signalling events before the activation of PKC and/or intracellular calcium mobilization.
K Ogasawara; H Tomioka; T Shimizu; C Sano; H Kawauchi; K Sato
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  129     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-07     Completed Date:  2002-09-11     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  272-80     Citation Subset:  IM    
Department of Microbiology and Immunology, Shimane Medical University, Izumo, Shimane, Japan.
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MeSH Terms
Cell Communication / immunology
Immune Tolerance
Macrophages / enzymology,  immunology*
Mice, Inbred BALB C
Mice, Inbred C57BL
Mycobacterium avium Complex / immunology*,  pathogenicity*
Mycobacterium avium-intracellulare Infection / immunology*
Protein Kinase C / metabolism
Signal Transduction
T-Lymphocytes / immunology
T-Lymphocytes, Regulatory / immunology
Reg. No./Substance:
EC Kinase C

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