Document Detail

Profile of toll-like receptor-positive cells in septic and aseptic loosening of total hip arthroplasty implants.
MedLine Citation:
PMID:  20127718     Owner:  NLM     Status:  MEDLINE    
Distinction between the two major complications of total hip replacement surgery, septic bacterial culture-positive and aseptic bacterial culture-negative osteolysis and loosening, is difficult due to the eventual role of bacterial remnants and biofilms, which are recognized by cells provided by toll-like receptors (TLRs) of the innate immune system. It was hypothesized that cell typing and TLR mapping might provide new information on the pathomechanisms of loosening. To test this hypothesis, septic (n = 10) and aseptic (n = 5) interface tissue as well as mildly inflamed osteoarthritic synovial membrane (n = 5) samples were characterized and compared using antibodies against several cell line-specific markers, including fibroblast, monocyte/macrophage, T cell, B cell, plasma cell and neutrophil markers, and TLRs. In osteoarthritic synovium, TLR-positive cells were restricted to surface tissues and only few inflammatory cells were detected, whereas aseptic interface was heavily infiltrated with monocyte/macrophages, which were also the major TLR-positive cell type rendering the tissue reactive to TLR ligands. Interestingly, septic cases contained also neutrophil and lymphocyte infiltrates of which especially B-cell infiltrates might be clinically useful in discriminating the two major complications of the joint replacement surgery. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.
Jukka Pajarinen; Elisabetta Cenni; Lucia Savarino; Enrique Gomez-Barrena; Yasunobu Tamaki; Michiaki Takagi; Jari Salo; Yrjö T Konttinen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  94     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-08-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  84-92     Citation Subset:  IM    
Department of Medicine, Biomedicum Helsinki, Helsinki University Central Hospital, P.O. Box 700, FIN-00029, Helsinki, Finland.
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MeSH Terms
Aged, 80 and over
Arthroplasty, Replacement, Hip*
Biological Markers / metabolism
Middle Aged
Osteoarthritis / immunology,  pathology
Osteolysis / metabolism,  pathology
Prosthesis Failure*
Sepsis / complications*,  immunology
Synovial Membrane / immunology,  microbiology,  pathology
Toll-Like Receptors / immunology*
Reg. No./Substance:
0/Biological Markers; 0/Toll-Like Receptors

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