| Profibrotic Cytokine TGF-β1 Increases Endothelial Progenitor Cell Angiogenic Properties. | |
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MedLine Citation:
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PMID: 22284809 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: TGF-β1 is a profibrotic cytokine that plays a major role in vascular biology, and is known to regulate the phenotype and activity of various vascular cell populations. Because most fibrotic diseases, such as Idiopathic Pulmonary Fibrosis (IPF), are associated with vascular remodeling and since endothelial progenitor cells may be involved in this process, we investigated the impact of TGF-β1 modulation of endothelial progenitor cell angiogenic properties. Patients/ methods: TGF-β1 plasma levels were determined in 64 patients with IPF and compared to controls. The effect of TGF-β1 on angiogenesis was studied in vivo in a Matrigel plug model and in vitro on Endothelial Colony Forming Cells (ECFCs). We studied the effects of inhibiting the expression of the three main receptors of TGF-β1 in ECFCs using siRNA. Results: Total TGF-β1 plasma levels were significantly increased in patients with IPF compared to controls (P < 0.0001). TGF-β1 had proangiogenic effects in vivo by increasing hemoglobin content and blood vessels formation in Matrigel-plugs implanted in C57/Bl6 mice and in vitro by enhancing ECFC viability and migration. The effects were abolished by silencing the three main TGF-β1 receptors. Conclusions: TGF-β1 is pro-angiogenic in vivo and induces ECFC angiogenic properties in vitro, suggesting that TGF-β1 may play a role during vascular remodeling in fibrotic disease states via endothelial progenitor cells. |
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Authors:
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S M Evrard; C d'Audigier; L Mauge; D Israël-Biet; C L Guerin; I Bieche; J C Kovacic; A-M Fischer; P Gaussem; D M Smadja |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-27 |
Journal Detail:
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Title: Journal of thrombosis and haemostasis : JTH Volume: - ISSN: 1538-7836 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101170508 Medline TA: J Thromb Haemost Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 International Society on Thrombosis and Haemostasis. |
Affiliation:
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Université Paris Descartes, Paris INSERM UMRS 765, Paris AP-HP, Hôpital Européen Georges Pompidou, Service d`Hematologie Biologique, Paris AP-HP, Hôpital Européen Georges Pompidou, Service de Pneumologie INSERM UMRS 970, Paris Cardiovascular Research Center - PARCC, Paris INSERM UMRS 745, Paris Laboratoire d'Oncogénétique, Centre René Huguenin, Saint Cloud, France The Zena and Michael A Wiener Cardiovascular Institute, Mount Sinai School of Medicine, New York, NY Vascular Biology Program and Surgery department, Children`s Hospital, Harvard Medical School, Boston, MA, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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