|The profibrotic cytokine transforming growth factor-β1 increases endothelial progenitor cell angiogenic properties.|
|PMID: 22284809 Owner: NLM Status: MEDLINE|
|BACKGROUND: Transforming growth factor-β1 (TGF-β1) is a profibrotic cytokine that plays a major role in vascular biology, and is known to regulate the phenotype and activity of various vascular cell populations. Because most fibrotic diseases, such as idiopathic pulmonary fibrosis (IPF), are associated with vascular remodeling, and as endothelial progenitor cells (EPCs) may be involved in this process, we investigated the impact of TGF-β1 modulation of EPC angiogenic properties.
METHODS: TGF-β1 plasma levels were determined in 64 patients with IPF and compared with those in controls. The effect of TGF-β1 on angiogenesis was studied in vivo in a Matrigel plug model and in vitro on endothelial colony-forming cells (ECFCs). We studied the effects of inhibiting the expression of the three main receptors of TGF-β1 in ECFCs by using short interfering RNA.
RESULTS: Total TGF-β1 plasma levels were significantly increased in patients with IPF as compared with controls (P < 0.0001). TGF-β1 had proangiogenic effects in vivo by increasing hemoglobin content and blood vessel formation in Matrigel plugs implanted in C57/Bl6 mice, and in vitro by enhancing ECFC viability and migration. The effects were abolished by silencing the three main TGF-β1 receptors.
CONCLUSIONS: TGF-β1 is proangiogenic in vivo and induces ECFC angiogenic properties in vitro, suggesting that TGF-β1 may play a role during vascular remodeling in fibrotic disease states via EPCs.
|S M Evrard; C d'Audigier; L Mauge; D Israël-Biet; C L Guerin; I Bieche; J C Kovacic; A-M Fischer; P Gaussem; D M Smadja|
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|Type: Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't|
|Title: Journal of thrombosis and haemostasis : JTH Volume: 10 ISSN: 1538-7836 ISO Abbreviation: J. Thromb. Haemost. Publication Date: 2012 Apr|
|Created Date: 2012-04-02 Completed Date: 2012-08-31 Revised Date: 2014-09-09|
Medline Journal Info:
|Nlm Unique ID: 101170508 Medline TA: J Thromb Haemost Country: England|
|Languages: eng Pagination: 670-9 Citation Subset: IM|
|© 2012 International Society on Thrombosis and Haemostasis.|
|Data Bank Information|
Bank Name/Acc. No.:
|APA/MLA Format Download EndNote Download BibTex|
Aged, 80 and over
Endothelial Cells / metabolism*
Fetal Blood / cytology
Hemoglobins / metabolism
Idiopathic Pulmonary Fibrosis / blood, metabolism*, physiopathology
Mice, Inbred C57BL
Receptors, Transforming Growth Factor beta / genetics, metabolism
Stem Cells / metabolism*
Transforming Growth Factor beta1 / blood, metabolism*
|0/Hemoglobins; 0/Receptors, Transforming Growth Factor beta; 0/TGFB1 protein, human; 0/Transforming Growth Factor beta1|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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