Document Detail

Proenkephalin gene expression is altered in the brain of spontaneously hypertensive rats during the development of hypertension.
MedLine Citation:
PMID:  7968371     Owner:  NLM     Status:  MEDLINE    
Enkephalins have been discovered in various regions of the brain involved in cardiovascular regulation. Sympathoadrenal hyperactivity and altered baroreflex activity have been implicated in the development of hypertension. The purpose of this investigation was to determine whether proenkephalin gene expression is altered in the arterial baroreceptor reflex region of the brain and in neurons involved in regulating sympathetic outflow, during the development of hypertension. Proenkephalin mRNA levels were compared, using in situ hybridization, in 4- and 14 week-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Systolic blood pressure was measured by tail-cuff impedance plethysmography. There were no differences in blood pressure at 4 weeks, however by 14 weeks resting systolic blood pressure was approximately 40% higher in SHR (162.5 +/- 1.6 vs. 117.3 +/- 1.5 mmHg). Proenkephalin gene expression in the nucleus tractus solitarius (NTS), caudal (CVLM) and rostral ventrolateral medulla (RVLM) was lower (approximately 67, 50, and 55%, respectively) in the SHR at 14 weeks. However, in the locus coeruleus (LC), anterior (AH) and lateral hypothalamus (LH), proenkephalin mRNA was significantly increased (approximately 50, 100 and 100%, respectively) in the SHR. The decrease in proenkephalin mRNA in the NTS, CVLM, and RVLM may attenuate arterial baroreceptor reflex activity, while the increase in proenkephalin mRNA in the LC, AH and LH may increase sympathetic tone by inhibiting the activity of sympathodepressor preganglionic neurons in the intermediolateral cell column of the spinal cord.
J B Boone; D McMillen
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  24     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-12-07     Completed Date:  1994-12-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  320-6     Citation Subset:  IM    
University of Missouri, Department of Veterinary Biomedical Sciences, Columbia 65211.
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MeSH Terms
Aging / metabolism*
Blood Pressure
Body Weight
Brain / growth & development,  metabolism*
Enkephalins / biosynthesis*
Gene Expression*
Hypertension / metabolism,  physiopathology*
In Situ Hybridization
Oligonucleotide Probes
Organ Specificity
Protein Precursors / biosynthesis*
RNA, Messenger / analysis,  metabolism
Rats, Inbred SHR / physiology*
Rats, Inbred WKY / physiology
Grant Support
Reg. No./Substance:
0/Enkephalins; 0/Oligonucleotide Probes; 0/Protein Precursors; 0/RNA, Messenger; 0/proenkephalin

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